The potential Anti-Inflammatory and antioxidant activities of Proanthocyanidins in acetic acid–induced ulcerative colitis in rats | ||
| Benha Veterinary Medical Journal | ||
| Article 48, Volume 37, Issue 1, September 2019, Pages 245-252 PDF (973.14 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2019.14356.1036 | ||
| Authors | ||
| Samy Ali Hussein1; Omayma A.R. Abou Zaid2; Samir Abdel Latif3; Anhar Mohamed EL-Sebaey* 4 | ||
| 1Department of Biochemistry, Faculty of Vet. Med., Benha University, Egypt. | ||
| 2Clinical Biochemistry Department, Faculty of Veterinary Medicine, Benha University, Egypt | ||
| 3Department of Animal Hygiene, Behavior and Management, Faculty of Veterinary Medicine, Benha University, Egypt | ||
| 4Biochemistry,veterinary,benha,benha,egypt | ||
| Abstract | ||
| Objective: The present study was designed to investigate the anti-colitic effect of Proanthocyanidins (Pcs) on acetic acid -induced ulcerative colitis in rats. Methods: Forty male rats (220-250 g) were divided into five equal groups. Group Ι:(Control normal group) rats received no drugs. Group Π: (Early UC-induced group) rats received 3% acetic acid (2ml/rat) intracolonially and sacrificed 3 days later from induction. Group III:(Late UC –induced group) rats received acetic acid similar to group 2 and sacrificed after 21 days. Group IV:(Early UC+Pcs protected group) rats received Pcs (200 mg/kg body weight/day) orally for 21 days prior to acetic acid administration for UC induction. Group V:(Late UC+Pcs treated group) treated with Pcs as in group III for 21 days after UC induction. Results: A significant increase in L-MDA and MOP with marked decrease in GSH and CAT activity in colon of acetic acid -induced colitis in rats as compared with control group. However, a significant depletion of colon tissue L-MDA, MOP and marked increase in GSH and CAT activity were observed after Pcs treatment compared to ulcerated untreated rats. The qPCR results revealed a significant upregulation of mRNA gene expression levels of TNF-α, COX-2 and a significant downregulation in TGF-β1 in colon of acetic acid -induced UC in rats. The expression levels of TNF-α, COX-2 was significantly downregulated and a significant upregulated in TGF-β1 in colon tissues after administration of Pcs. Conclusions: Proanthocyanidins protects rats colon mucosa damage against acetic acid -induced ulcerative colitis via anti-inflammatory and anti-oxidative mechanisms. | ||
| Keywords | ||
| Proanthocyanidins; acetic acid; Colitis; anti-inflammatory; transforming growth factor | ||
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