Metabolomics and Bioactivity Guided Isolation of Secondary Metabolites from the Endophytic Fungus Chaetomium sp.
Tawfik, N., Tawfike, ., Abdo, R., Abbott, G., Abdelmohsen, U., Edrada-Ebel, R., Haggag, E. (2017). Metabolomics and Bioactivity Guided Isolation of Secondary Metabolites from the Endophytic Fungus Chaetomium sp.. EKB Journal Management System, 1(1), 66-74. doi: 10.21608/aprh.2016.933
Nashwa Tawfik; ِAhmed Tawfike; Randa Abdo; Grainne Abbott; Usama Ramadan Abdelmohsen; RuAngelie Edrada-Ebel; Eman Haggag. "Metabolomics and Bioactivity Guided Isolation of Secondary Metabolites from the Endophytic Fungus Chaetomium sp.". EKB Journal Management System, 1, 1, 2017, 66-74. doi: 10.21608/aprh.2016.933
Tawfik, N., Tawfike, ., Abdo, R., Abbott, G., Abdelmohsen, U., Edrada-Ebel, R., Haggag, E. (2017). 'Metabolomics and Bioactivity Guided Isolation of Secondary Metabolites from the Endophytic Fungus Chaetomium sp.', EKB Journal Management System, 1(1), pp. 66-74. doi: 10.21608/aprh.2016.933
Tawfik, N., Tawfike, ., Abdo, R., Abbott, G., Abdelmohsen, U., Edrada-Ebel, R., Haggag, E. Metabolomics and Bioactivity Guided Isolation of Secondary Metabolites from the Endophytic Fungus Chaetomium sp.. EKB Journal Management System, 2017; 1(1): 66-74. doi: 10.21608/aprh.2016.933

Metabolomics and Bioactivity Guided Isolation of Secondary Metabolites from the Endophytic Fungus Chaetomium sp.

Journal of Advanced Pharmacy Research
Article 6, Volume 1, Issue 1, January 2017, Page 66-74  XML PDF (976.68 K)
Document Type: Research Article
DOI: 10.21608/aprh.2016.933
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Authors
Nashwa Tawfik1; ِAhmed Tawfike1; Randa Abdo1; Grainne Abbott2; Usama Ramadan Abdelmohsen3; RuAngelie Edrada-Ebel2; Eman Haggag email 1
1Pharmacognosy Department, Faculty of Pharmacy, Helwan University
2Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Scotland, United Kingdom
3Faculty of Pharmacy, Minia University, Minia, Egypt
Abstract
Objectives: the aim of this study is to explore the secondary metabolites produced by the endophytic fungus Chaetomium sp. isolated from Scencio stapeliiformis (E. Phillips) as well as investigate the anticancer and antimicrobial activity of crude extracts, fractions and pure compounds. Methods: An endophytic fungus (Chaetomium sp.) was isolated from the aerial part of S. stapeliiformis (from Giza, Egypt). DNA sequencing analysis, morphological and chemotaxonomy investigations were used for taxonomic identification. Metabolomics tools and dereplication studies were employed to choose the optimum growth medium and conditions that produce the most significant metabolites. The crude extract of the optimal fungal culture of Chaetomium sp. was then fractionated using flash chromatography and medium pressure liquid chromatography (MPLC). The structure of the isolated compounds was determined on the basis of 1D, 2D NMR and mass spectrometry (HR-ESIMS) analysis. Results: The bioassay-guided isolation afforded five pure compounds; p-hydroxybenzaldehyde (1), Uracil (2), 3-benzyl-6-isobutyl piperazine-2,5-dione (3), Cyclo (L-Alanin-L-leucin) (4) and Cyclo-(L-proline-L-leucine) (5). Multivariate data analysis highlighted the most significant metabolites contributed to the measured bioactivity. All fungal extracts were tested for the anticancer activity but only 30 days LC extract of Chaetomium was active. The pure compounds were tested for their anticancer and antimicrobial activities. Compounds 3 and 5 exhibited a significant anti-trypanosomal activity while compounds 1, 2 and 5 effectively inhibited the growth of E-coli and Staphylococcus aureus. Conclusion: A combination of metabolomic- and bioassay-guided protocol can efficiently predict the putative biologically active metabolites during the first stage of fractionation.
Keywords
antimicrobial activity; antitrypanosomal activity; Chaetomium sp; Dereplication; Endophytes; metabolomics; Senecio stapeliiformis
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