A SIMPLE TECHNIQUE FOR STAINING OF PLATYHELMINTHS WITH THE LACTOPHNOL COTTON BLUE STAIN | ||
| Journal of the Egyptian Society of Parasitology | ||
| Article 13, Volume 43, Issue 2, August 2013, Pages 419-423 PDF (317.3 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jesp.2013.94819 | ||
| Authors | ||
| ADAWIA A.M. HENEDI; OSAMA M.E. EL-AZAZY | ||
| Veterinary Laboratories, Public Authority of Agriculture Affairs and Fish Resources, P. O. Box 21422, Safat 13075, Kuwait. | ||
| Abstract | ||
| This paper describes a simple technique for staining of flatworms using lactophenol cotton blue (LPCB). The staining was tested on 2 trematode species: Heterophyes heterophyes and Mesostephanus appendiculatus, and one cestode: Diplopylidium acanthotetra, which were collected from the intestine of stray cats in Kuwait. The specimens were mounted in a small amount of the LPCB stain on a clean slide for 2-3 minutes before covering with a cover slip. The technique rapidly and clearly differentiated the internal structures of the helminthes. Its speed and simplicity are advantages over other staining methods. It is easily used in wide-scale surveys where a large number of platyhelminths have to be identified and it is suitable for field studies. | ||
| Keywords | ||
| LPCB stain; flatworms; cats; Kuwait | ||
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