Effects Of Dietary Application Of Two Antagonistic Gut-Isolated Bacillus Species On The Immune Response Of Oreochromis niloticus To Aeromonas hydrophila Infection | ||
| Zagazig Veterinary Journal | ||
| Article 4, Volume 41, Issue 2, June 2013, Pages 31-39 PDF (3.57 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zvjz.2013.95681 | ||
| Authors | ||
| ZM El-Bouhy; GA El-Nobi; ME Hassanin; MA El-Hady | ||
| Dept. of Fish Diseases and Management, Faculty of Veterinary Medicine, Zagazig University, Egypt | ||
| Abstract | ||
| Two Bacillus species isolates (B1 and B2),showed in-vitro antagonistic activity against Aeromonas hydrophila isolated from the intestinal tract of apparently healthy Oreochromis niloticus. Both antagonistic isolates were identified as Bacillus licheniformis and Bacillus cereus/thuringiensis, respectively by using Biolog’s microbial identification system, and they were evaluated to be safe to O. niloticus when inoculated intra-peritoneal (I/P). Feeding experiments were carried out in-vivo to investigate the effect of both antagonistic isolates (1 X 107 CFU/g diet) either alone or combined on fish immune response and resistance to A. hydrophila infection. Immunological parameters (phagocytic, lysozyme and serum bactericidal activities) were evaluated as well as a challenge test using pathogenic A. hydrophila. Results revealed that, antagonistic Bacillusisolates either alone or combined triggered significant (P ≤ 0.05) increase in phagocytic, lysozyme and serum bactericidal activities; with the highest values in fish received a mixture of both antagonistic isolates. Survival of A. hydrophila challenged fish was highest in fish fed on both antagonistic isolates, followed by B. cereus/thuringiensis, and then B. licheniformis fed fish. | ||
|
Statistics Article View: 203 PDF Download: 257 |
||
