Biodegradation of Malachite Green by White-Rot Fungus, Pleurotus Pulminarious | ||||
| Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||||
| Article 8, Volume 12, Issue 1, June 2020, Page 79-90 PDF (1.1 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsg.2020.96943 | ||||
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| Authors | ||||
| Nuha Amao Afibirin Zubbair1; Abdullahi Taiwo Ajao2; Elijah Olaolu Adeyemo3; Omoyele Dele ADENIYI4 | ||||
| 1Department of Science Laboratory Technology, Microbiology Unit, Institute of Pure and Applied Sciences, Kwara State Polytechnic, Ilorin. | ||||
| 2Department of Biosciences and Biotechnology, College of Pure and Applied Sciences, Kwara Stae University, Malete, Nigeria | ||||
| 3Department of Science Laboratory Technology, Chemistry/Biochemistry Unit, Institute Of Pure and Applied Sciences, Kwara State Polytechnic, Ilorin | ||||
| 4Cocoa Research Institute of Nigeria,Ibadan, Oyo State, Nigeria | ||||
| Abstract | ||||
| Wide spectrum of the industrial applications of Malachite green dyes and its lethal effect on the living organisms become major source of concerns as this causes damage to tissues, hindered growth and development system, developmental abnormalities, and mutagenic/carcinogenic potentials. This study evaluated the degradation of malachite green by laccase producing Pleurotus pulminarious. The pH, Total Protein, and laccase activities were used to monitor the progress of the degradation. The laccase activity and protein contents increased as the biodegradation process progressed with the highest Laccase activities and extracellular protein values of 0.2867±0.00 and 14.872±0.43 respectively at pH 3.9 and decreased progressively with increase in pH. Metabolites obtained after decolorizations were analyzed with GC-MS and FTIR studies and confirmed the biodegradation of dye. GC-MS analysis identified metabolites that were produced after the degradation of MG. This study demonstrated complete degradation of MG into low molecular weight compounds such as 3-Benzylhexahydropyrrolo[1,2-a] pyrazine-1,4-dione,3-Isobutylhexahydropyrrolo [1,2-a] pyrazine-1,4-dione, 3-Aminoacetophenone, N,4,4-Trimethylcyclohexanamine, 2,3,4-Trimethylhexane. It was concluded that the organism posses multifunction of having medicinal values, production of industrially important laccase, and for the treatment of industrial effluent containing malachite green. | ||||
| Keywords | ||||
| Ecofriendly; Malachite green; Laccase; Biodegradation; Pleurotus pulminarious | ||||
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