STABILITY OF Staphylococcus aureus BIOFILM AGAINST DIFFERENT CHEMICAL AGENTS AND SOME ENZYMES | ||
| Zagazig Journal of Agricultural Research | ||
| Article 18, Volume 43, Issue 5, September 2016, Pages 1699-1704 PDF (144.29 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zjar.2016.98129 | ||
| Authors | ||
| Belal A.M. Omar* ; M. Z. Sitohy; R. A. El-Massray; A. M. Abo Etta | ||
| Biochem. Dept., Fac. Agric., Zagazig Univ., Egypt | ||
| Abstract | ||
| The Biofilm of Staphylococcus aureus was tested against a various of substances at different concentrations for 12 hr., to show its stability. The chelating agent Ethylene diamine tetra acetic acid (EDTA) (1-5%) didn't show any effect on the biofilm stability. Oxidizing agents also didn`t show a negative effect on the biofilm stability. By adding enzymes like Pepsin and Papain (100-500µl) the stability of biofilm did not affect. Organic solvents e.g.; hexan, toluene and methyl alchohol added at 1-5% also did not destabilize the biofilm stability. Acids and basics like (HCl and NaOH) were added with concentrations of 1-5%. The effect of different substance on the stability of biofilm were very slight. Adjusting the bacterial media to pH 2-13 using HCl and NaOH before the biofilm formation was effected and showed positive effect and prevent biofilm formation between pH (2-5) and pH (10-13). | ||
| Keywords | ||
| Staphylococcus arues; biofilm; stability | ||
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