Preparation and Evaluation 0f A Recent Infectious Laryngotracheitis (LIT) Vaccine From A Local Field Isolate | ||||
| Journal of Applied Veterinary Sciences | ||||
| Article 10, Volume 5, Issue 3, July 2020, Page 87-91 PDF (476.58 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/javs.2020.101927 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Nada A. Fathy  1; Abd El-Moneam M.M.1; Ibrahim S.2; Nourhan Nagy Mohamed3; Amina Radwan4; Rafik Hamed Sayed5
| ||||
| 1Department of Newcastle disease vaccine research, Veterinary Serum and Vaccines Research Institute (VSVRI), Agricultural Research Center (ARC), Abbasia, Cairo, Egypt. | ||||
| 2Department of Genetic Engineering, Veterinary Serum and Vaccines Research Institute (VSVRI), Agricultural Research Center (ARC), Abbasia, Cairo, Egypt | ||||
| 3Central Laboratory for Evaluation of Veterinary Biologics (CLEVB)El-Seka El-Baida, Abbasia, Cairo, Egypt | ||||
| 4Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center (ARC), Abbasia, Cairo , Egypt. | ||||
| 5Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center (ARC), Abbasia, Cairo , Egypt | ||||
| Abstract | ||||
| Infectious laryngotracheitis (ILT) is a respiratory tract disease affecting chickens worldwide. The disease leads to severe production losses due to increased mortality, decreased egg production, and delayed body weight gain, causing enormous economic losses to the poultry industry. ILT is controlled through vaccination with live-modified attenuated vaccines of chicken embryo origin (CEO) and/or the tissue-culture origin (TCO). This study was conducted to develop Specific Pathogenic Free (SPF) egg-adapted live attenuated ILT vaccine from the field strain Fayoum_2018 isolated from broilers in Fayoum Province, Egypt in 2018. The isolate Fayoum_2018 with accession number (MN082684) was characterized molecularly as a TCO vaccine related strain. Measurement of immunity in vaccinated chicks employing the potency test revealed that chicks vaccinated with 0.5 ml of prepared ILT vaccine exhibited ELISA with 1905 antibody titer at 2nd-week post-vaccination, which was considered an ILT adequate immune response and increased till reach 3497 antibody titer at 6th-week post-vaccination. The current work revealed that Fayoum_2018 ILTV egg adapted live attenuated vaccine produced a satisfactory antibodies titer that was efficient in controlling the ILT field infection in Egypt. | ||||
| Keywords | ||||
| BVDV; ILT; CEO; TCO; SPF; ELISA | ||||
| References | ||||
|
ANDREASEN J.R, GLISSON J.R, GOODWIN M.A, RESURRECCION R.S, VILLEGAS P AND BROWN J. 1989. Studies of infectious laryngotracheitis vaccines: immunity in layers. Avian Dis., 33: 524–530.
Brandly, CA and Bushnell, L.D. 1934. A report of some investigations of infectious laryngotracheitis. Poultry Science, 13, 212–217.
CFR (CODE OF AMERICAN FEDERAL REGULATION-13CFR) USA 2017. Published by the office of the federal register national archives records service, Animals and animal products Ch.111.30-11333.
DAVISON, A.J. 2010. Herpes virus systematics. Veterinary Microbiology, 143, 52–69.
FULTON, R.M., SCHRADER, D.L. AND WILL, M.2000. Effect of route of vaccination on the prevention of infectious laryngotracheitis in commercial egg-laying chickens. Avian Diseases, 44: 8–16.
GUY J.S., BARNES H.J. AND MORGAN L.M. 1990. Virulence of infectious laryngotracheitis viruses: Comparing modified-live vaccine viruses and North Carolina field isolates, Avian Dis, 34:106-113.
GUY, J. AND GARCÍA, M. 2008. Laryngotracheitis. In YM Saif, A.M. Fadly, J.R. Glisson, L.L. McDougald, L.K. Nolan and D.E. Swayne,(Eds.).Diseases of Poultry 12th Edn. (pp. 137–152). Ames: Blackwell.
HAN, M.G. AND KIM, S.J.2003. Efficacy of live virus vaccines against infectious laryngotracheitis assessed by polymerase chain reaction-restriction fragment length polymorphism. Avian Diseases, 47: 261–71.
MAY H.G AND TITTSLER R.P .1925. Tracheo-laryngotracheitis in poultry. J Am Vet Med Assoc, 67: 229-231.
NOURHAN NAGY, SHERIF MAHMOUD, AMINA RADWAN, RAFIK HAMED, MAHMOUD ABD EL-MONEIM, NADA ADEL, HANAN MOHAMED EL-ZAHED .2019. Isolation and molecular characterization of circulating infectious laryngotracheitis (ILT) virus in Egypt, j.Egypt.vet.med.Assoc 79, no 3. 743 – 759.
OIE MANUAL .2018.Avian Infectious Laryngo-tracheitisOIE TERRESTRIAL MANUAL. 2014. Chapter 2.3.3 Avian Infectious Laryngo-tracheitisPALOMINO-TAPIA V.A, ZAVALA G, CHENG S, AND GARCÍA. M .2019. Long-term protection against a virulent field isolate of infectious laryngotracheitis virus induced by inactivated, recombinant, andmodified live virus vaccines in commercial layers. Avian Pathol., 48 (3): 209-220.
REED, L.J. AND MUENCH, H. 1938. "Simple method of estimating 50 percent endpoint", Am. J. Hyg. , 27: 493-499.RODRI´GUEZ-AVILA, A, I. OLDONI, I., RIBLET, S. M. AND GARCI´A, M. 2008. Evaluation of the protection elicited by direct and indirect exposure to live attenuated infectious laryngotracheitis virus (ILTV) vaccines against a recent challenge strain from the United States. Avian Pathology, 37, 287-292.
SHAN-CHIA OU, JOSEPH J GIAMBRONE .2012. Infectious laryngotracheitis virus in chickens. World J Virol 2012 October 12; 1(5): 142-149
VAGNOZZI, A., ZAVALA, G, RIBLET, S.M., MUNDT, A., GARCIA, M. 2012. Protection induced by commercially available live attenuated and recombinant viral vector vaccines against infectious laryngotracheitis virus in broiler chickens. Avian Pathol. 41, 21-31. | ||||
|
Statistics Article View: 581 PDF Download: 801 |
||||
