RHIZO-FILTRATION OF CU FROM WATER VIA WATER LETTUCE (PISTIA STRATIOTS) USING NA- LAURYL SULPHATE SURFACTANT

الفلترة الجذریة  للنحاس من المیاه بواسطة نبات خس الماء

باستخدام کبریتات الصودیوم لوریل

مجدى محمد نیازی

معهدبحوثالاراضی والمیاه والبیئه-مرکزالبحوث الزراعیه- الجیزه- مصر

العناصر الثقیلة  من الملوثات التی یصعب ازالتها من البیئه حیث لاتخضع للتکسیر الکیمیائى أو البیولوجى توجد  عدة طرق لازالة العناصر الثقیله من میاة الصرف الصناعى او الزراعى الملوثه ولکنها ذات تکلفه مرتفعه  لکن یمکن ازالة التلوث باستخدام النباتات المائیة الطافیة مثل خس الماء

تم  اجراء تجربة استخدم فیها النبات الطافى خس الماء عن طریق نموالجذور فی اصص تملأ محالیل مغذیة  بها ترکیزات متزایدة من الکادمیوم  هی  0  و 5 و 10 و 15  و 20   ملیجرام لتر فى المحلول المغذى (حجم 5 لتر)وقد اضیف النحاس فی صورة  کبریتات النحاس (Cu So4, 5 H2O) وذلک لمدة  5و10 و15 و20 یوم

کان نمو النبات 21.90 ، 17.00 ، 14.61 ، 12.39 ، و 10.52جرام لکل اصیص لعلاج 0 ، 5 ، 10 ، 15 و 20 ملغ من CuL-1 فی غیاب کبریتات الصودیوم لوریل (SLS) على التوالی ، وعلى الجانب الآخر من اضافة کبریتات صودیوم لوریل SLS ظهر تأثیر سلبی کبیر على إنتاج الکتلة الحیویة.

حدثت إزالة النحاس من المیاه الملوثة مع کل المعاملات  بالنحاس بدون SLS. وکانت المتوسطات 489.03 ، 575.51 ، 644.68 و 693.42 میکروغرام من النحاس لکل اصیص عند معاملات 5،10،15 و 20 ملجرام نحاس لکل لتر على التوالی. کانت عملیات الإزالة الناتجة عن امتصاص الجذر 76.75 و 106.43 و 132.11 و 195.75 میکرو جرام لکل اصیص  على التوالی.

أدت إضافة SLS فی المیاه الملوثة إلى زیادة إزالة النحاس حیث ان تأثیر المخلبى لمادة SLS ادى الى ان النحاس اقابل للذوبان ومتاح بسهولة أکبر. وبالتالی ، کانت ترکیزات النحاس فی  الاصص المحتویة على نبات  خس الماءغیر المعالجة (بدون SLS) منخفضة ، فی حین أن النباتات المعالجة SLS کانت قادرة على استخراج کمیات کبیرة من النحاس. توصى الدراسة  باستخدام مخلب SLS لازالة مع نبات خس الماء لانه یجعل النحاس متاحًا بسهولة لخس الماء (Pistiastratiots).

RHIZO-FILTRATION OF CU FROM WATER VIA WATER LETTUCE (PISTIA  STRATIOTS)  USING NA- LAURYL SULPHATE SURFACTANT

Magdy M. Niazy

Soil, Water and Environ. Res. Inst., Agric. Res. Cent. (ARC), Giza, Egypt

Key words:Rhizofiltration ,water lettuce (Pistiastratiots) ,Na- lauryl sulphate surfactant , and copper 

Abstract

Rhizofiltrationwas used to decrease   Cuin   water having  5 , to  20 mg Cu L^-1   using water lettuce (Pistiastratiots)  in  water culture (5-L pot capacity ), with a contact time of  5, 10, 15 and 20 days.Weight of Plant growth  was 21.90 , 17.00, 14.61, 12.39 ,and 10.52 for treatments of  0 , 5 , 10, 15 and 20 mg CuL^-1  in absence of Sodium Lauryl Sulfate (SLS) respectively. SLS treatment showed negative effect on the biomas production. Removal of Cu from water occurred with all Cu-treaments without SLS. Averages  were 489.03, 575.51, 644.68 and 693.42   ug Cu pot^-1   for treatments of  5,10,15 and 20  mg pot^-1 respectively. Respective removals caused by root uptake were 76.75, 106.43, 132.11 and 195.75 ug  pot^-1  respectively. Cu in shoots ranged from 28 to 61 ugCu g^-1 and in roots the range was 35to 150 ug Cu g^-1 dry matter. Adding SLSto water increased the removal of Cu. The chelating effect of the SLS made the metal more easily available. Copper in untreated plants (no SLS) were low, while the SLS- treated plants were capable of extracting large amounts of copper. The chelating effect of the SLS on metal increased its availability to water lettuce (Pistiastratiots).

INTRODUCTION

In Egypt, pollution of the river Nile water hasincreased in the past few decades because of increased populationand activities along the Nile,and increased area of arable land increased  pollution (Fawzy et al., 2012).Phytoremediation is based on utilization of plants to decontaminate soil andwater (Sherameti and Varma, 2015,  Bonanno et al., 2017 and Kumar et al., 2017).Using living plants to remove metals fromcontaminated water (rhizofiltration or phytofiltration)are phytoremediation processes (Espinoza-Quinones et al., 2008& 2009 and Abdelsalam et al., 2015).The removal of contaminants in surface water by plant rootsinvolves adsorption or precipitation of elements onto plant roots or absorption followed by sequestration in roots. This process occurs with Pb, Cd, Cu, Fe, Ni,Mn, Zn and  Cr as well as  radionuclide ⁹⁰Sr, ¹³⁷ Cs, ²³⁸ U, ²³⁶ U (Dushenkov et al.,1995and1997a & b).Aquatic plants  are used in water quality assessmentas bio-monitors (sentinel species),and phyto-remediators to remove suspended solids(Lytle and Lytle, 2001).The ability of aquatic macro-phytes to uptake nutrients directly from water bodies and  assimilate them is of  great benefit (Galal and Shehata 2014). Several aquatic plants effective in heavy metal uptake have been identified (Khankhane et al., 2014). Aquatic plant-based treatment systems are low-cost technologies, which can be adopted by developing countriesfor recycling/treatment of wastewaters, especially those contaminatedby toxic metals (Fawzy et al., 2012). Recent studies concluded that aquatic  macrophytes including water lettuce (Pistiastratiotes), water  hyacinth (Eichhorniacrassipes), and duckweed (Lemma gibba) can be effectively used to capture many pollutants including heavy metals from polluted waters (Miretzky et al., 2004&2006; Odjegba and Fasidi 2004 andOlguin et al. 2017). Water lettuce (Pistiastratiotes) species of family Araceaeis used in phytoremediation. Roots of this floating   plant   hang   underneath their leaves (Williams and Hecky, 2005 and Gupta et al., 2012).The plant displays high  capacityin taking up  impurities, with  extensive spreading roots. It is inexpensive for propagation, and its tissues and cells can be examined microscopically in observing the contaminants (El-Gendy et al., 2005 andDipu et al., 2011).In tropical or subtropical areas, the plant  is used in phytoremediation water systems (Silva et al., 2013). In comparison with inherent plants, it shows greater mineral elimination competence, high uptake  capability and rapid growth(Irfan, 2015). It proved effective in accumulating  heavy metals from wastewaters in Egypt (Galal and Farahat 2015 and Galal et al., 2018).It grows in slow-flowing canals in north Nile Delta, and in Embaba near Cairo (Boulos 2005)as well as  in stagnant waters, around Fariskur , north Delta (Tackholm 1974). It grows  in Lake Mariut (Galal and Farahat 2015) and Lake Manzala (Galal et al., 2012).

Sodium Lauryl Sulphate (SLS) ,Sodium Dodecyl Benzene Sulphate (SDBS) andSodium TtriPolyPhosphate(STPP)]surfactants, are used in numerous industrial applications including  food, paints, plastics, pharmaceuticals, cosmetics, and textiles  (Cserháti et al., 2002). In particular, anionic surfactants are popular detergent ingredients, because of their synthesis and low production costs. As a consequence of their widespread use and strong resistance to biodegradation,surfactants may persist in wastewater treatment systems at relativelyhigh concentrations (Dirilgen and Ince 1995 andPetterssonet al., 2000).They are used for their ability to meliorate the solubility of petroleum hydrocarbons and heavy metals such as Cd, Zn, Cu and Pbin polluted soils, hence increasing their removal by leaching (Torres et al., 2007 and Ramamurthy and Schalchian 2013) They are used in combination with phytoremediation with herbaceousplants(Liu et al., 2008, 2009, Almeida et al., 2009,Almansoory et al., 2015 and Mao et al., 2015).

The objective of the current study is to assess the phyto-extraction effectiveness of water lettuce (Pistiastratiots) using Na- lauryl sulphate surfactant

MATERIALS AND METHODS

Pot experiments were conducted to evaluate the efficiency of water lettuce (Pistiastratiotes L.) for phytoremediation of copper contaminated water. Two experiments were  done. One experiment was conducted without presence of Sodium Lauryl Sulfate (SLS). The second was conducted in presence of SLS { CH3(CH2)11(OCH2CH2)nOSO3Na , a relative molar mass of 420 g mol-1 } at 0.5 mM pot^-1  (Liu et al. 2008.  The experimental design for each experiment was a randomized complete block, factorial , with 3 replicates . The 2 factors were as follows: Factor 1:  Cu content in water; with 5 treatments i.e.  0, 5, 10, 15 and 20mg Cu L^-1 in the form of copper sulphate (Cu SO₄, 5 H₂O).Factor 2:   Time of exposure duration of roots into the water culture with 4 treatments i.e. 5, 10, 15 and 20 days. Therefore the treatment combinations were 20, and the total number of  treatments was 60. Each pot (PVC) was 20-cm diameter; 40-cm height was filled with 5 L Hoagland nutrient solution maintaining pH between 7.1-7.4. .The pH of the culture water was maintained at between 6.0 to 6.5 using 0.1M HCl and 0.1M NaOHWahla and Kirkham, (2008) andRolli etal.,(2017) Fig 1 shows a drawing of the set-up. The plants were collected from at El-Serw village, south of Manzalah Lake, north eastern Egypt (longitude 31^-: 45⁰–32^-–50⁰ E and latitude 31^-: 00–31^-: 35⁰ N), and identified according toTackholm (1974). Fresh and healthy plants of approximately same size and weight were selected, washed with distilled water rinsed with deionized water (Yusuf et al., 2002). The experiments were done during summer of 2019 at the Experimental Farm of Kafr El-Hamam Agricultural Research  Station El Sharkia Governorate, Egypt. This site is located at 30° -35 N latitude and 30° - 57 E   longitudes with an elevation of about 7 meters above mean sea level. Plants were put in a Hoagland solution, for a 10-day acclimatization period, before subjection to heavy metal contamination.  There were two plants per potCopper sulphate (Cu So₄, 5 H₂O)was usedat concentrations of 0, 5, 10, 15 and 20 mg L^-1 in absence and presence of Sodium Lauryl Sulfate (SLS) .Times of assessing effects were  5, 10, 15 and 20days . The volume of water in each pot was kept constant and the change in volume due to evapotranspiration was compensated by addition of deionized water. ؛ CuCu, was determined after digestion with  perchloric, nitric and sulfuric acid mixture (Stewart,1989). Water and plant samples were analyzed for Cu. The removal efficiency was calculated following equation Ganjo and Khwakaram2010 and Kumar et al., 2017):                

Where R is the removal efficiency in percent (%), while C₀and C_t refer to the initial and residual (after t days) heavymetals concentrations in the aqueous solution (mg L⁻¹),respectively.

Fig 1:Ascimatic drawing  rhizo-filtration set up

Removal of copper from water was calculated as follows:

R= (C₁) - (C₀)

-where R =The removal of Cu from water culture

-,C ₁= Final concentration of copper in plants after exposure and C₀=Concentration of copper in plants before exposure

RESULTS AND DISCUSSION

Plant growth:

Plant growth increased with time; the 20 days growth was 9.37% greater (on average) than the 5 days growth (Tables 1to 4and Fig2&3). On the other hand the growth decreased with increased concentration of Cu in water exhibiting a retarding effect by Cu without SLS. Weights of plant growth (roots+shoots)were  21.90 , 17.00, 14.61, 12.39 ,and 10.52 for treatments of  0 , 5 , 10, 15 and 20 mg CuL^-1  respectively , exhibiting a progressive decrease with the increase in Cu  in the culture water . Howevercopper with SLS (Sodium Lauryl Sulfate)decreased were 21.90,14.42,11.09,8.23 and6.25 respectively for the same abovementioned parameters with copper rates of 0 , 5 , 10, 15 and 20 mg CuL^-1.The biomass of plants was determined as dry weight.The weight decreased with the increase in the concentration of copper without SLS.This is due to increased Cu in Pistiastratoiteswhich caused oxidative stress and decreased photosynthesis and, .Same studies showed that1900mgL^-1 of Cu in hydroponic cultures caused leaf chlorosis in Arabidopsis thaliana (Li et al., 2008).Other studies showed that 491mg Cu L^-1, decreased maize root weight in water culture by 50%(Ouzounidou et al.,1995).Increased contents of Cuin culture solution caused decreases in growth weight of plant. These results agree with the findings of Jovanic et al., (2010)who reported that SLS decreased the photosynthetic rate and chlorophyll content in bean plants.

Table 1 : Using water lettuce (Pistiastratiots)  to remove Cu from water culture: shoots&roots dry weight (g pot-1) with exposure for 5,10,15 and 20 days without SLS.

Red= reduction %={(control weight – treatment weight)/control weight} x 100

Fig 2: Using water lettuce (Pistiastratiots)  to remove Cu from water culture: shoots&roots  dry weight (g pot-1) with exposure for 5,10,15 and 20 days without SLS

Table 2 : Using water lettuce (Pistiastratiots)  to remove Cu from water culture: shoots&roots  dry weight (g pot-1) with exposure for 5,10,15 and 20 days withSLS.

Fig 3: Using water lettuce (Pistia  stratiots)  to remove Cu from waterculture:shoots&roots  dry weight (g pot-1) with exposure for 5,10,15 and 20 days withSLS

Table3: Usingwater lettuce (Pistiastratiots)) to remove CUfrom water culture: Growth dry weight of shoots  and roots  as  affected by water Cu  without SLS   .

Table 4: Using water lettuce (Pistiastratiots)) to remove Cu from water culture: Growth dry weight of shoots  and roots  as  affectedby water Cu  with SLS

Removal of Cd from the water culture:

Plants removed Cu without and with SLS from the water culture (Tables 5and 6). Plants grown in the no-Cu showed no detectable Cu.The average removal of Cu through uptake over the 5 Cu treatments by plant (shoots+roots) were 110.46,489.03, 575.51, 644.68 and 693.42   ug Cu pot^-1   for 5Cutreatments (average of the four immersion times)  . Comparable uptakes by roots were 7.43, 76.75, 106.43, 132.11 and 195.75 ug pot^-1 respectively. The  progressive decrease  is in line with the  increase  in  Cu in  growth media  the decreased plant   growth  associated  with the increased Cu  in the water culture. Contents of Cu in root were generally greater than in shoots. Contents in roots averaged 35, 57, 85 and 150 ugg-¹ (an average of 82 ug g^-1) . Comparable contents in shoots were 28, 37, 48 and 61 ug g-1 (average of 44 ug g^-1) without SLS addition.

 As for the effect of copper with SLS application the obtained data clearly indicate that the average removal of Cu through uptake amounts by plant (shoots+roots) were 110.46,641.03,691.73,745.12 and 808.79 ug Cu pot^-1 for 5 treatments respectively while uptakes by roots were7.43, 93.76, 120.97, 153.85and182.43 ug pot^-1 for the 5 Cu treatments respectively.

The decrease plant growth cause by Cu addition is in harmony with results of Vinodet al., 2019obtained decreased plant growthP. stratiotes plants upon addition of 5 to 20mg Cu L^-1 in water culture solutionJiang et al., (2000) showed that root biomass of BrasicaJunceadecreased  with increasing, Cu contamination. The negative effect of Cu on plant growth was reported in plants such as maize (Liu et al., 2001) andwheat (Cook et al., 1997and Dudka et al., 1994).

Table 5 :Using water lettuce (Pistiastratiots) to remove Cufrom water culture: Cu removal  by plants (ug pot-1) with exposure for 5,10,15 and 20 days without SLS

Table 6 :Using water lettuce (Pistiastratiots) to remove Cufrom water culture: Cu removal  by plants (ug pot-1) with exposure for 5,10,15 and 20 days with SLS

Negative effect of Cu could be due to interference with metabolic processes associated with normal development (Lidon and Henriques, 1992;  VanArshe and Clijster, 1990).Cu decreased  chlorophyll as well as leaf water content. The decrease inChlorophyll may be due to inhibition of chlorophyll synthesis and protochlorophyllidereductase activity (Stiboravaet al., 1987) and stimulation of chlorophyll- degrading chlorophyllase activity (Drazkiewice, 1994). It is likely that the decrease in leaf water content RWC was mainly due to on the plasma membrane permeability of cells (Ohsumi et al., 1988).The SLS treatment caused negativeeffect on the plant growth. These results agree with findings of Sharrel et al., 2014 who noted negative effect SLS, lowering the pH wouldincreases the toxic effect of metal pollution.SLS decreased  photosynthetic rate and chlorophyll content in bean plants Jovanic et al. (2010)and Gadallah(1996).Noted a retarding effect of SLSonsunflower. Effectivemetal removal of heavy metals by aquatic plants was reported by many researchers with removal rates close to or higher than 90% (Mishra and Tripathi, 2008 andMungur et al., 1997).High metal removal rates are common when aquatic plants contains high concentrations of metals (Kao et al., 2001).

Metals such as Zn, Mn, Ni and Cu are essential micronutrients  andtheir, accumulation does not exceed their metabolic needs of <10mg Kg^-1(Boyd and Martens, 1994).Water lettuce can be a hyperaccumulator of Heavy metals(Zayed et al., 1998)and its usein remediating  waste waterscan be effective(Mishra and Tripathi, 2008). Results of the present are in harmony with those recorded byVardanyan and Ingole, (2006) who found that roots of water lettuce had high Cu content than shootsSLS can be used to in remediation of somehydrocarbons(Mulligan et al., 1999a,b).Pants respond to heavy metals by ways  including immobilization, exclusion, compartmentaliziation and  synthsis of metallothioneins, (Sanita-Di-Toppiand Gabbrielli, 1999 and Abdelsalamet al., 2015).

According to Bernard, (1997).mechanisms of copper tolerance in higher plants can be grouped as follows:

a)      Exclusion or restriction of copper uptake

b)      .Immobilization of Cu in cell walls.

c)      Compartmentation of Cu in insoluble complexes.

d)     Compartmentation of Cu in soluble complexes.

e)      Enzyme adaptation

It may be conclude that rhizofiltration can be applicable and appropriate at low cost. Selection of the appropriate plant species is vital. Based on the present results Pistiastratoites may be used to remove Cu from contaminated waters. Phytoremediation of wastewater through rhizofiltration using Pistiastratoites can be pracical preposition with low costs.

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الفلترة الجذریة  للنحاس من المیاه بواسطة نبات خس الماء

باستخدام کبریتات الصودیوم لوریل

مجدى محمد نیازی

معهدبحوثالاراضی والمیاه والبیئه-مرکزالبحوث الزراعیه- الجیزه- مصر

العناصر الثقیلة  من الملوثات التی یصعب ازالتها من البیئه حیث لاتخضع للتکسیر الکیمیائى أو البیولوجى توجد  عدة طرق لازالة العناصر الثقیله من میاة الصرف الصناعى او الزراعى الملوثه ولکنها ذات تکلفه مرتفعه  لکن یمکن ازالة التلوث باستخدام النباتات المائیة الطافیة مثل خس الماء

تم  اجراء تجربة استخدم فیها النبات الطافى خس الماء عن طریق نموالجذور فی اصص تملأ محالیل مغذیة  بها ترکیزات متزایدة من الکادمیوم  هی  0  و 5 و 10 و 15  و 20   ملیجرام \ لتر فى المحلول المغذى (حجم 5 لتر)وقد اضیف النحاس فی صورة  کبریتات النحاس (Cu So4, 5 H2O) وذلک لمدة  5و10 و15 و20 یوم

کان نمو النبات 21.90 ، 17.00 ، 14.61 ، 12.39 ، و 10.52جرام لکل اصیص لعلاج 0 ، 5 ، 10 ، 15 و 20 ملغ من CuL-1 فی غیاب کبریتات الصودیوم لوریل (SLS) على التوالی ، وعلى الجانب الآخر من اضافة کبریتات صودیوم لوریل SLS ظهر تأثیر سلبی کبیر على إنتاج الکتلة الحیویة.

حدثت إزالة النحاس من المیاه الملوثة مع کل المعاملات  بالنحاس بدون SLS. وکانت المتوسطات 489.03 ، 575.51 ، 644.68 و 693.42 میکروغرام من النحاس لکل اصیص عند معاملات 5،10،15 و 20 ملجرام نحاس لکل لتر على التوالی. کانت عملیات الإزالة الناتجة عن امتصاص الجذر 76.75 و 106.43 و 132.11 و 195.75 میکرو جرام لکل اصیص  على التوالی.

أدت إضافة SLS فی المیاه الملوثة إلى زیادة إزالة النحاس حیث ان تأثیر المخلبى لمادة SLS ادى الى ان النحاس اقابل للذوبان ومتاح بسهولة أکبر. وبالتالی ، کانت ترکیزات النحاس فی  الاصص المحتویة على نبات  خس الماءغیر المعالجة (بدون SLS) منخفضة ، فی حین أن النباتات المعالجة SLS کانت قادرة على استخراج کمیات کبیرة من النحاس. توصى الدراسة  باستخدام مخلب SLS لازالة مع نبات خس الماء لانه یجعل النحاس متاحًا بسهولة لخس الماء (Pistiastratiots).