Three Simple and Economic Spectrophotometric Methods for Simultaneous Determination of Chitosan and Ascorbic Acid
Al-Afify, N., Trabik, Y., Magdy, N., El-Kosasy, A. (2020). Three Simple and Economic Spectrophotometric Methods for Simultaneous Determination of Chitosan and Ascorbic Acid. EKB Journal Management System, 4(1), 92-103. doi: 10.21608/APS.2020.2004.1031
Nourhan Al-Afify; Yossra Trabik; Nancy Magdy; Amira El-Kosasy. "Three Simple and Economic Spectrophotometric Methods for Simultaneous Determination of Chitosan and Ascorbic Acid". EKB Journal Management System, 4, 1, 2020, 92-103. doi: 10.21608/APS.2020.2004.1031
Al-Afify, N., Trabik, Y., Magdy, N., El-Kosasy, A. (2020). 'Three Simple and Economic Spectrophotometric Methods for Simultaneous Determination of Chitosan and Ascorbic Acid', EKB Journal Management System, 4(1), pp. 92-103. doi: 10.21608/APS.2020.2004.1031
Al-Afify, N., Trabik, Y., Magdy, N., El-Kosasy, A. Three Simple and Economic Spectrophotometric Methods for Simultaneous Determination of Chitosan and Ascorbic Acid. EKB Journal Management System, 2020; 4(1): 92-103. doi: 10.21608/APS.2020.2004.1031

Three Simple and Economic Spectrophotometric Methods for Simultaneous Determination of Chitosan and Ascorbic Acid

Archives of Pharmaceutical Sciences Ain Shams University
Article 8, Volume 4, Issue 1, June 2020, Page 92-103  XML PDF (1.05 MB)
Document Type: Original Article
DOI: 10.21608/APS.2020.2004.1031
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Authors
Nourhan Al-Afify email 1; Yossra Trabikorcid 2; Nancy Magdy3; Amira El-Kosasy2
1Pharmaceutical analytical chemistry department, Faculty of pharmacy,Ain Shams University,Cairo,Egypt
2Pharmaceutical analytical chemistry department,Faculty of pharmacy,Ain Shams University,Cairo,Egypt
3Pharmaceutical analytical chemistry department,Faculty of pharmacy Ain shams University,Cairo,Egypt
Abstract
Three simple, precise, and sensitive UV spectrophotometric methodshave been developed and validated for the determination of Chitosan (CH) in the presence of Ascorbic acid (AA) in a binary mixture. Method A is a derivative ratio method (1DD) that measures the peak amplitude of the first derivative for CH and AA at 207.2 and 282 nm; respectively. Method B is a ratio difference method (RDSM) that measures the peak amplitude difference of the ratio spectra (DP204.6- 202.9) and (DP268-230) for CH and AA; respectively. Method C is a mean centering of ratio spectra method (MCR) that measures CH and AA at 204.6 and 269.1 nm; respectively. Method A, B, and C were able to successfully determine the studied drugs in a concentration range of 0.5-9 mg/mL for CH and 2-20 mg/mL for AA. All results were statistically compared with the reported methods, where no significant difference was observed. The proposed methods were applied to the analysis of the studied drugs in pure and pharmaceutical formulations.
Keywords
Chitosan; Functional polymers; Spectrophotometry; Derivative ratio method (1DD); Ratio difference method (RDSM); Mean centering of ratio spectra method (MCR)
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