Effect of Various Bicarbonate Supplements on Biodiesel Production and Valuable Biochemical Components of the Marine Eustigmatophyceae Nannochloropsis oculata (Droop) | ||||
| Egyptian Journal of Botany | ||||
| Article 35, Volume 60, Issue 3, December 2020, Page 785-796 PDF (1.14 MB) | ||||
| Document Type: Regular issue (Original Article) | ||||
| DOI: 10.21608/ejbo.2020.27849.1492 | ||||
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| Authors | ||||
Ali M. Abugrara1; Hanan M. Khairy  2; Heba S. El-Sayed3; Hoda H. Senousy4
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| 1Marine Resources Department, Faculty of Natural Resources and Environmental Sciences, Omar Al-Mokhtar University, El-Beda, Libya | ||||
| 2Hydrobiology Laboratory, Marine Environmental Division, National Institute of Oceanography and Fisheries Kayet Bay, Alexandria, Egypt | ||||
| 3Fish Reproduction, Larval rearing Laboratory (Marine Hatchery), National Institute of Oceanography and Fisheries, Kayet Bay, Alexandria, Egypt | ||||
| 4Botany and Microbiology Department, Faculty of Science, Cairo University, Giza 12613, Egypt | ||||
| Abstract | ||||
| MICROBIAL breeding media must be cost-effective, enable high growth, meet exact requirements and be readily available. The effect of different levels of sodium bicarbonate (SB) [25, 50, 75 and 100%] in the growth medium on the biochemical constituents (protein, carbohydrates, lipids, fatty acids, and amino acids) of the Nannochloropsis oculata was assessed compared to the F/2 Guillard standard medium. The obtained results revealed that the chemical constituents of N. oculata were influenced by the used level of sodium bicarbonate. The highest total protein, carbohydrate contents, and the maximum percentage of essential amino acids (EAA) (59.68%) were obtained by using the B1 medium (25% SB) as compared to the control (100% F/2). The highest total lipid content was achieved by using the B4 medium (100% SB) producing (20.09 %). In accordance, the highest total saturated fatty acids percentage (TSFA) of N. oculata was recorded by B4 medium. However, the highest total unsaturated fatty acids percentage (USFA) was exhibited by the B1 medium. The EN 14214 and ASTM D-6751 analyzes of the production of biodiesel have shown that the produced biodiesel by B4 medium (100% SB) and the control media of high quality. In conclusion, the addition of sodium bicarbonate is an excellent policy to increase chemical composition and lipid accumulation. The present study recommended taming results for either biodiesel production or aquaculture feeding by using proposed B4 medium as a lipid promoter or B1 medium as a protein promoter. | ||||
| Keywords | ||||
| Amino acids; Biodiesel production; Fatty acids; Nannochloropsis oculata ; Proximate composition | ||||
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