AN INVESTIGATION ON THE BIOCONVERSION 0F REFUSED RICE GRAINS TO ETHANOL | ||||
Misr Journal of Agricultural Engineering | ||||
Article 21, Volume 28, Issue 4, October 2011, Page 1119-1140 PDF (659.88 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/mjae.2011.102627 | ||||
View on SCiNiTO | ||||
Authors | ||||
M. M. Naim1; M. E. Yehia2 | ||||
1Prof., Chem. Eng. Dept., Faculty of Engineering. Alexandria University, Alexandria, Egypt. | ||||
2Res., Rice Technology Training Center, Field Crops Research Institute, A.R.C., Alexandria, Egypt. | ||||
Abstract | ||||
Technology for production of bio-ethanol has been developed during the last two decades to the point at which large-scale production will be a reality in the next few years. This is to produce source of energy from some agricultural waste origin. The various raw materials used in the manufacture of ethanol via fermentation are conveniently classified into three main types of raw materials: sugars, starches and cellulosic materials. The objective of the present research is aimed at producing bio-ethanol by hydrolysis of refuse broken rice grains. Four procedures were attempted, of which the last one was the best and the maximum concentration reached of ethanol was 58.5 %. The broken rice grains were washed, dried and milled to a very fine product, followed by adjustment of pH with dilute acid, then cooked in a batch pressure cooker in slurry form. The slurry is then treated with several fungi to hydrolyze starch to dextrins, after which the mash is treated with a second fungus to reduce the dextrins to monosaccharides. The product was then fermented by baker’s yeast saccharomyces cerevisiae (SC) into alcohol, the concentration of which was estimated by density determination of the clear filtrate at different time intervals. In order to separate the alcohol fraction, the liquid product can be either distilled or preferably subjected to separation by pervaporation (Pn) using different membranes of our choice which are fabricated in our lab. Dehydration or separation is accomplished by the use of the most suitable membrane with which the desired separation takes place. Furthermore, the by-product left over can be further processed to reduce its water content then sold as animal food. | ||||
Keywords | ||||
rice; saccharification; monosaccharides; fermentation; bio-ethanol; bioconversion; fungi; yeast | ||||
References | ||||
Aiba S, Shoda M (1969) Reassessment of the product inhibition in alcohol fermentation. J. Ferment Technol. 47:790-803.
Aiba S, Shoda M, Nagatani M (1968) Kinetics of product inhibition in alcoholic fermentation. Biotechnology Bioengineering 11: 846-864.
Akoh, C.C.; Chang, S-W.; Lee, G-C.; Shaw, J-F. (2008) Biocatalysis for the Production of Industrial Products and Functional Foods from Rice and Other Agricultural Produce, J. Agric. Food Chem., Vol. 56, 10445-10451.
Azmi, A.; Hasan, M.; Mel, M.; Ngoh, C. (2009) Single-step bioconversion of starch to bioethanol by the coculture of ragi tapai and Saccharomyces cerevisiae, Chemical Engineering Transaction Vol. 18, 557-562.
Batie,C.J.; Crabb,G.; Aux,G.W.; Cates,E.S.; Dinwiddie,J.A; Silverstone,A.L.; Quadt,R.; Miller,C.A." ,Process for Starch Liquefaction and Fermentation", Syngenta Biotechnology, INC.;Patent Department, US 2009/0047382 A1 .
Chen, J-P.; Wu, K-W.; Fukuda, H. (2008) Bioethanol production from uncooked raw starch by immobilized surface-engineered yeast cells, Appl Biochem. Biotechnol, 145, 59-67.
Choi,G-W.; Kang,H-W.; Kim,Y-R.; Chung,B.W. (2008) Ethanol production by Zymomonas mobilis CHZ2501 from industrial starch feedstocks, Biotechnology and Bioprocess Engineering, vol.13, 765-771.
EgamberdievNB, Jerusalimsky A (1968) Continuous cultivation of microorganisms. Czechoslovak Academy of Sciences, Prague.
Gervais P, Sarrette M (1990) Influence of age of mycelia and water activity on arpma production by Trichoderma viride. J Ferment Bioeng 69:46-50.
Gilson, C.D., Thomas, A., (1995) Ethanol production by alginate immobilized yeast in fluidized bed bioreactor. Journal of Chemical Technology and Biotechnology 62, 38-42.
Giordano, R.L.C.; Trovati,J.; Schmidell,W. (2008) Continuous Production of Ethanol from Starch Using Glucoamylase and Yeast Co-Immobilized in Pectin Gel, 147, 47-61.
Gong CS, Maun CM, Tsao GT (1981) Direct fermentation of cellulose to ethanol by a cellulolytic filamentous fungus Monilia ap. Biotechnol Lett 3:77-82.
Gulnur B, Pemra D, Betul K, Ilsen O, Kutlu U (1998) Mathematical description of ethanol fermentation by immobilized Saccharomyces Cererisiae. Process biochem 33(7):763-771.
Herrero AA, Gomez RF (1980) Development of ethanol tolerance in Clostridium thermocellum: effect of growth temperature. Appl Biochem Biotechnol 34/35:639-649.
Hinshelwood CN (1946) Kinetics of the bacterial cell. OxfordUniversity Press. London, UK.
Holzberg I, Finn RF, Steinkraus KH (1967) A hinetic study of the alcoholic fermentation of grape juice. Biotechnol Bioeng 9:413-423.
Hoppe GK, Hansford GS (1982) ethanol inhibition of continuous anaerobic yeast growth. Biotechnol Lett 4: 39-44.
Ingram LO, Conway T, Clark DP, Sewell GW, Preston JF (1987) Genetic engineering of ethanol production in Escherichia coli. Appl Environ Microbiol 53:2420-2425.
Ito K, Yoshida K, Ishikawa T, Kobayaashi S (1990) Volatile compounds produced by fungus Aspergillus oryzae in rice koji and their changes during cultivation. J Ferment Bioeng 70:169-172.
J. C. du Preez, Fiona de Jong, P. J. Botes and P. M. Lategan (2007 ) Ethanol fermentation technologies from sugar and starch feed stocks , Elsevier Inc., vol.26, 89-105.
Lee, C.G.; Kim, C.H., Rhee, S.K. (1991) A kinetic model and simulation of starch saccharification and simultaneous ethanol fermentation by amyloglucosidase and Zymomonas mobilis, Bioprocess and biosystems engineering, vol.7, 335-341.
Lin, Y.; Tanaka, S. (2006) Ethanol fermentation from biomass resources: current state and prospects, Appl Microbiol Biotechnol, 627–642.
Luong JHT (1985) Kinetics of ethanol inhibition in alcohol fermentation. Biotechnol Bioeng 27:280-285.
Maia, A.B.R.A., Nelson, D.L., (1993) Application of gravitational sedimentation to efficient cellular recycling in continuous alcoholic fermentation. Biotechnology and Bioengineering 41, 361-369.
McGHEE, J. E.; CARR, M. E.; JULIAN, G. ST. (1984) Continuous Bioconversion of Starch to Ethanol by Calcium-Alginate Immobilized Enzymes and Yeasts, The American Association of Cereal Chemists. Inc. VOI.61, No.5, 446-449.
Mori A, Konno N, Terui G (1970) Kinetic studies on submerged acetic acid fermentation. I. Behaviors of Acetobacter rancens cells towards dissolved oxygen. J Ferment Technol 48:203-212.
Mori, Y; Inaba, (2005) Ethanol production from starch in a pervaporation membrane bioreactor using Clostridium thermohydrosulfuricum, Wiley Periodicals, Inc., 36, 849 – 853.
Nagatani M, Shoda M, Aiba S (1968) Kinetics of product inhibition in alcoholic fermentation. J Ferment Technol 46:241-249.
Naim, M. M.; and Yehia, M. E.; (2009) A novel and simple technique for the determination of quantity of alcohol produced in wet paddy rice due to fermentation Misr Journal of Agricultural Engineering. Vol. 26 No. (4).
Namba A, Tamura A, Nagai S(1984) Synergistic effects of acetic acid and ethanol on the growth of Acetobacter sp. J Ferment Technol 62(6):501-505.
Nikoli, S.; Mojovi,L.; Pejin,D.; Rakin,M.; Vucurovic,v. (2009) Improvement of Ethanol Fermentation of Corn Semolina Hydrolyzates with Immobilized Yeast by Medium Supplementation , Food Technol. Biotechnol, vol. 47, 83–89.
Ochoa, S.; Yoo, A.; Repke, J-U.; Wozny, G.; Yang, D.R. (2008) Modeling and identification of the bio-ethanol production process from starch: Cybernetic vs. unstructured modeling, Elsevier B.V., vol. 25, 707-712.
Oh KK,Kim SW, Jeong YS, Hong SI (2000) Bioconversion of cellulose into ethanol by nonisothermal simultaneous saccharification and fermentation. Appl Biochem Biotechnol 89:15-30.
Park YS, Kiyoshi T, Fukaya M, Okumura H, Kawamura Y (1991) Production of a high concentration acetic acid by Acetobacter aceti using a repeated fed-batch culture with cell recycling. Appl Microbiol Biotechnol 35:149-153.
Park YS, Ohtake H, Toda K (1990) A kinetic study of acetic acid production by liquidsurface culture of Aceetobacter aceti. Appl Microbiol Biotechnol 33:259-263.
Pastore GM, ParkYK, Min DB (1994) Production of a fruity aroma by Neurospora from beiju. Mycol Res 98: 25-35.
Perry, R. H.," Perry's chemical engineer's handbook", McGraw hill, 7TH edition, 1979.
Roy, I; Gupta,M.N. (2003) Hydrolysis of starch by a mixture of glucoamylase and pullulanase entrapped individually in calcium alginate beads ,Elsevier Inc.,VOL.34, 26-32.
Roychoudhury PK, Ghose TK, Ghosh P (1992) Operational strategies in vacuum-coupled SSF for conversion of lignocellulose th ethanol. Enzyme Microb Technol 14:581-585.
Saddler JN, Chan MKH (1982) Optimization of Clostridium thermocellum growth on cellulose and pretreated wood substrates .Eur J Appl Microbiol Biotechnol 16:99-10-4.
Sugawara E, Hashimoto S, Sakurai Y, Kobayashi A(1994) Formation by yeast of the HEMF (4-hydrpxy-2 (or 5)-ethyl-5 (or 2)-methyl-3 (2H)-furanone) aroma components in Miso with aging. Biosci Biotechnol Biochem 58:1134-1135.
Suresh,K.; Kiransree,N.;Rao,L.V.(1999) Production of ethanol by raw starch hydrolysis and fermentation of damaged grains of wheat and sorghum, Bioprocess and Biosystems Engineering, vol.21, 165-168.
Wukovits,W.; Pfeffer,M.; Liebmann,B.; Friedl,A (2008) Integration of the bio-ethanol process in a network of facilities for heat and power production from renewable sources using process simulation, Elsevier B.V., vol. 24, 1295-1300. | ||||
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