Neuroprotective Effect of Combined Therapy of Liraglutide with Vitamin D on Streptozotocin-Induced Diabetic Neuropathy in Rats.
ElBadre, H., ElBadre, M., Mostafa, M., Sedeek, M. (2020). Neuroprotective Effect of Combined Therapy of Liraglutide with Vitamin D on Streptozotocin-Induced Diabetic Neuropathy in Rats.. EKB Journal Management System, 24(2), 118-136. doi: 10.21608/smj.2020.28543.1149
Hala Mostafa ElBadre; Mohamed Mostafa ElBadre; Mohamed Ahmed Mostafa; Mona Hassan Sedeek. "Neuroprotective Effect of Combined Therapy of Liraglutide with Vitamin D on Streptozotocin-Induced Diabetic Neuropathy in Rats.". EKB Journal Management System, 24, 2, 2020, 118-136. doi: 10.21608/smj.2020.28543.1149
ElBadre, H., ElBadre, M., Mostafa, M., Sedeek, M. (2020). 'Neuroprotective Effect of Combined Therapy of Liraglutide with Vitamin D on Streptozotocin-Induced Diabetic Neuropathy in Rats.', EKB Journal Management System, 24(2), pp. 118-136. doi: 10.21608/smj.2020.28543.1149
ElBadre, H., ElBadre, M., Mostafa, M., Sedeek, M. Neuroprotective Effect of Combined Therapy of Liraglutide with Vitamin D on Streptozotocin-Induced Diabetic Neuropathy in Rats.. EKB Journal Management System, 2020; 24(2): 118-136. doi: 10.21608/smj.2020.28543.1149

Neuroprotective Effect of Combined Therapy of Liraglutide with Vitamin D on Streptozotocin-Induced Diabetic Neuropathy in Rats.

Sohag Medical Journal
Article 19, Volume 24, Issue 2, April 2020, Page 118-136  XML PDF (516.36 K)
Document Type: Original Article
DOI: 10.21608/smj.2020.28543.1149
View on SCiNiTO View on SCiNiTO
Authors
Hala Mostafa ElBadre email 1; Mohamed Mostafa ElBadre2; Mohamed Ahmed Mostafa3; Mona Hassan Sedeek4
1Medical Biochemistry, Faculty of Medicine, Assuit University
2Department of Medical Pharmacology, Faculty of Medicine, Assiut University, Assiut, Egypt.
3Anaesthesia, ICU and Pain Relief Department, South Egypt Cancer Institute, Assuit University, Egypt.
4Department of Physiology, Faculty of Medicine, Aswan University, Aswan, Egypt.
Abstract
ABSTRACT

Background:
Liraglutide is an incretin mimetic agent that approved recently in type-2 diabetes. The use of vitamin D is associated with an improvement in diabetic neuropathy.
The aim of the study:
Evaluation of the effect of liraglutide and vitamin D each alone and in combination with each other on diabetic neuropathy induced by streptozotocin in rats.
Materials and Methods:
Five groups ofWistar rats were used in the experiment. Diabetic neuropathy was induced in groups 2, 3, 4, and 5 using a single intraperitoneal(i.p.) injection of streptozotocin(STZ) in a dose of 60 mg/kg. The third, fourth, and fifth groups were treated for 4 weeks with liraglutide (0.8 mg/kg), vitamin D3 (12 µg/kg), and combination of the 2 drugs, respectively. Then the behavioral tests were done (hot plate, tail-flick, paw withdrawal pressure, and the rotarod tests). Blood samples were used for assessment of blood glucose, tumor necrosis factor -α (TNF-α) and interleukin-Iβ(IL-Iβ). Malondialdehyde(MDA) and glutathione (GSH)were measured in the sciatic nerve homogenate.
Results:
Liraglutide caused significant improvement in the behavioral tests of the diabetic rats with a significant reduction in blood glucose, TNF-α, IL-1β, and malondialdehyde. Vitamin D caused mild improvement in the behavioral tests, inflammatory and oxidative stress markers. The combined use of liraglutide with vitamin D caused more improvement in diabetic neuropathy tests, inflammatory and oxidative stress markers.
Conclusion:
Liraglutide has a moderate neuroprotective, anti-inflammatory, and antioxidant effects in cases of streptozotocin-induced diabetic neuropathy which are enhanced by the addition of vitamin D.
Keywords
Keywords: Diabetic neuropathy; Liraglutide; Streptozotocin; Vitamin D
Supplementary Files
Statistics
Article View: 388
PDF Download: 390