Bacteriological and Molecular diagnosis of Most Common Bacteria Causing subclinical Mastitis in Cow
Saad, W., Abd El-Tawab, A., Nabih, A. (2019). Bacteriological and Molecular diagnosis of Most Common Bacteria Causing subclinical Mastitis in Cow. EKB Journal Management System, 37(2), 28-32. doi: 10.21608/bvmj.2020.18716.1126
Walaa Saad; Ashraf Abd El-Tawab; Ashraf Nabih. "Bacteriological and Molecular diagnosis of Most Common Bacteria Causing subclinical Mastitis in Cow". EKB Journal Management System, 37, 2, 2019, 28-32. doi: 10.21608/bvmj.2020.18716.1126
Saad, W., Abd El-Tawab, A., Nabih, A. (2019). 'Bacteriological and Molecular diagnosis of Most Common Bacteria Causing subclinical Mastitis in Cow', EKB Journal Management System, 37(2), pp. 28-32. doi: 10.21608/bvmj.2020.18716.1126
Saad, W., Abd El-Tawab, A., Nabih, A. Bacteriological and Molecular diagnosis of Most Common Bacteria Causing subclinical Mastitis in Cow. EKB Journal Management System, 2019; 37(2): 28-32. doi: 10.21608/bvmj.2020.18716.1126

Bacteriological and Molecular diagnosis of Most Common Bacteria Causing subclinical Mastitis in Cow

Benha Veterinary Medical Journal
Article 5, Volume 37, Issue 2, December 2019, Page 28-32  XML PDF (1.05 MB)
Document Type: Original Article
DOI: 10.21608/bvmj.2020.18716.1126
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Authors
Walaa Saad email 1; Ashraf Abd El-Tawab2; Ashraf Nabih3
1Central Diagnostic & Research Lab, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt
2Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University
3Animal Reproduction Research Institute (ARRI), Ministry of Agriculture, Giza, Egypt
Abstract
The aim of the study was using polymerase chain reaction (PCR) technique for detecting the most bacteria causing mastitis in cows as streptococci spp., Escherichia coli and Staphylococcus aureus by using Genus specific primers for each one. One hundred milk samples were collected from cases of subclinical bovine mastitis positive for California Mastitis Test (CMT). The samples were bacteriologically cultivated on to 5% sheep blood agar and also, on different types of media (MacConkey's agar plates, Edward's agar medium and mannitol salt agar) to isolate the major bacteria causing mastitis and identified biochemically. Finally, these bacteria were identified by using PCR technique with a specific primer for each genus. The PCR results revealed that E. coli 16/46 (34.7%), Staphylococcus aureus (S. aureus) 12/40 (30%) and streptococci spp. 11/39 (28.2%). Multiplex PCR for typing of streptococci spp. revealed that St. agalactiae 3/39 (7.69%) and St. dysgalactiae 8/39 (20.51%). In conclusion, in the present study, PCR analysis was used for diagnosis of major bacteria causing subclinical mastitis in cows by using specific primers for each one. The most prevalent bacteria causing subclinical mastitis were E. coli, S. aureus, S. dysgalactia and S. agalactia.
Keywords
Bovine Mastitis; PCR; Staphylococcus aureus; streptococci spp; E. coli
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