Isolation and genetic analysis of BoHV-1 gC gene isolated from animals suffering respiratory disease in Dakahlia, Egypt 2018
Elashmawy, A., Ali, S., El-Habbaa, A., Bastawecy, I. (2019). Isolation and genetic analysis of BoHV-1 gC gene isolated from animals suffering respiratory disease in Dakahlia, Egypt 2018. EKB Journal Management System, 37(2), 23-27. doi: 10.21608/bvmj.2019.17295.1099
Asmaa Mounier Elashmawy; Saad Sharawi Ali; ayman said El-Habbaa; Iman Mohamed Bastawecy. "Isolation and genetic analysis of BoHV-1 gC gene isolated from animals suffering respiratory disease in Dakahlia, Egypt 2018". EKB Journal Management System, 37, 2, 2019, 23-27. doi: 10.21608/bvmj.2019.17295.1099
Elashmawy, A., Ali, S., El-Habbaa, A., Bastawecy, I. (2019). 'Isolation and genetic analysis of BoHV-1 gC gene isolated from animals suffering respiratory disease in Dakahlia, Egypt 2018', EKB Journal Management System, 37(2), pp. 23-27. doi: 10.21608/bvmj.2019.17295.1099
Elashmawy, A., Ali, S., El-Habbaa, A., Bastawecy, I. Isolation and genetic analysis of BoHV-1 gC gene isolated from animals suffering respiratory disease in Dakahlia, Egypt 2018. EKB Journal Management System, 2019; 37(2): 23-27. doi: 10.21608/bvmj.2019.17295.1099

Isolation and genetic analysis of BoHV-1 gC gene isolated from animals suffering respiratory disease in Dakahlia, Egypt 2018

Benha Veterinary Medical Journal
Article 4, Volume 37, Issue 2, December 2019, Page 23-27  XML PDF (1.23 MB)
Document Type: Original Article
DOI: 10.21608/bvmj.2019.17295.1099
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Authors
Asmaa Mounier Elashmawy email 1; Saad Sharawi Aliorcid 2; ayman said El-Habbaaorcid 3; Iman Mohamed Bastawecy4
1virology department, animal health institute
2Department of virology , faculty of veterinary medicine, benha university
3Department of Virology, Faculty of Veterinary Medicine, Benha University
4Virology Department, Animal Health Research Institute, Dokki, Giza, Egypt
Abstract
Objective of the present study was to isolate, identify and genetically characterize bovine herpes virus Type 1 (BoHV-1) from suspected cattle showing clinical symptoms of respiratory disease in Dakahlia, Egypt, September 2018.The virus from suspected nasal swabs and lung tissue samples was adapted onto specific pathogen free - embryonated chicken eggs (SPF - ECE).Pock lesions on the chorio-allantoic membrane (CAM) of SPF - ECE ranged from 1 to 2 mm in diameter and scattered all over the membrane that were more pronounced after the third passge. Sensitive PCR assay specific for BoHV-1 gC gene was also performed for molecular identification of the viral isolate and the gC PCR product was sequenced. Phylogenetic analysis of gC gene sequence identified and clustered our isolate with BoHV1.1. This highlights the isolation, rapid and sensitive detection of BoHV-1 virus from clinical cases and its subtyping by nucleotide sequencing and subsequent phylogenetic analysis which gives valuable information about the molecular epidemiology of BoHV-1 subtypes prevalent in the country.
Keywords
BoHV-1; ECE; PCR; sequence; phylogeny
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