Detection of virulence factors of Mycoplasma species isolated from chicken by multiplex PCR
El.ordy, M., Abd-El-Tawab, A., Hassan, W. (2020). Detection of virulence factors of Mycoplasma species isolated from chicken by multiplex PCR. EKB Journal Management System, 38(1), 61-65. doi: 10.21608/bvmj.2020.23772.1164
Mohammed El.ordy; Ashraf Abd-El-Tawab; Wafaa Hassan. "Detection of virulence factors of Mycoplasma species isolated from chicken by multiplex PCR". EKB Journal Management System, 38, 1, 2020, 61-65. doi: 10.21608/bvmj.2020.23772.1164
El.ordy, M., Abd-El-Tawab, A., Hassan, W. (2020). 'Detection of virulence factors of Mycoplasma species isolated from chicken by multiplex PCR', EKB Journal Management System, 38(1), pp. 61-65. doi: 10.21608/bvmj.2020.23772.1164
El.ordy, M., Abd-El-Tawab, A., Hassan, W. Detection of virulence factors of Mycoplasma species isolated from chicken by multiplex PCR. EKB Journal Management System, 2020; 38(1): 61-65. doi: 10.21608/bvmj.2020.23772.1164

Detection of virulence factors of Mycoplasma species isolated from chicken by multiplex PCR

Benha Veterinary Medical Journal
Article 12, Volume 38, Issue 1, March 2020, Page 61-65  XML PDF (219.36 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2020.23772.1164
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Authors
Mohammed El.ordy email 1; Ashraf Abd-El-Tawab2; Wafaa Hassan3
1Veterinarian
2Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University, Egypt.
3National Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Dokki-Giza, Egypt.
Abstract
One hundred and sixty clinically diseased broiler local chickens collected from 22 different farms belonging to Qalubia, Dakahlia and Gharbia governorates were subjected to bacteriological examination and molecular characterization. Clinical Signs were chronic respiratory disease with respiratory manifestations,lameness, loss or reduction in egg production.Bacteriological examination showed that 18.18% of the isolates were Mycoplasma positive and showed growth in pleuropneumonia-like organism (PPLO) agar plates, with fried egg appearance when examined by stereoscopic microscope. Mycoplasma colonies were tested for antimicrobial sensitivity tests against 12 antimicrobial antibiotics and showed higher sensitivity to nitrofurantoin, gentamicin, norfloxacin, ciprofloxacin and neomycin. Intermediate sensitivity were recorded to ceftriaxone and cefotaxime and antibiotic resistance were recorded to tetracyclines, lincomycin, chloramphenicole and sulphamethoxasine. Identification of the bacterial strains of the isolates were conducted by multiplex PCR by using two primer pairs for Mycoplasma synoviae (vlhA) and Mycoplasma gallisepticum (mgc2). The amplicons expected size were 396 bp, and 300 bp for vlhA and mgc2 respectively. Only13 out of 22 farms were positive, representing 59.09 %.Moreover, the incidence rate of Mycoplasma synoviae (vlhA) and Mycoplasma gallisepticum (mgc2) were 22.72% and 13.63% respectively and 22.72% of the inspected farms showed positive results for both Mycoplasma strains.
Keywords
MS (vlhA); MG (mgc2); PCR; broiler chickens; sensitivity test
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