HPLC Standardization of The Methanolic Extract of Acrocarpus fraxinifolius leaves based on Gallic acid Content
El-Nashar, H., Eldahshan, O., Singab, A. (2017). HPLC Standardization of The Methanolic Extract of Acrocarpus fraxinifolius leaves based on Gallic acid Content. EKB Journal Management System, 1(1), 5-8. doi: 10.21608/aps.2017.10356
Heba A.S. El-Nashar; Omayma A. Eldahshan; Abdel Nasser B. Singab. "HPLC Standardization of The Methanolic Extract of Acrocarpus fraxinifolius leaves based on Gallic acid Content". EKB Journal Management System, 1, 1, 2017, 5-8. doi: 10.21608/aps.2017.10356
El-Nashar, H., Eldahshan, O., Singab, A. (2017). 'HPLC Standardization of The Methanolic Extract of Acrocarpus fraxinifolius leaves based on Gallic acid Content', EKB Journal Management System, 1(1), pp. 5-8. doi: 10.21608/aps.2017.10356
El-Nashar, H., Eldahshan, O., Singab, A. HPLC Standardization of The Methanolic Extract of Acrocarpus fraxinifolius leaves based on Gallic acid Content. EKB Journal Management System, 2017; 1(1): 5-8. doi: 10.21608/aps.2017.10356

HPLC Standardization of The Methanolic Extract of Acrocarpus fraxinifolius leaves based on Gallic acid Content

Archives of Pharmaceutical Sciences Ain Shams University
Article 2, Volume 1, Issue 1, January 2017, Page 5-8  XML PDF (695.09 K)
Document Type: Original Article
DOI: 10.21608/aps.2017.10356
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Authors
Heba A.S. El-Nashar1; Omayma A. Eldahshan1; Abdel Nasser B. Singab email 2
1Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo, Abassia, 11566, Egypt
2Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo, 11566, Egypt.
Abstract
High-Performance Liquid Chromatography (HPLC) was developed for the standardization and quantification of gallic acid in the methanolic extract of Acrocarpus fraxinifolius Weight & Arn leaves. Linearity was observed in the range of 0.4–2 mg/mL with a correlation coefficient (r2) of 0.9978. The limit of detection (LOD) and limit of quantification (LOQ) were 0.0054 mg/mL and 0.0163 mg/mL, respectively indicating the sensitivity of the applied method. Recovery values of 100.377 % indicate the best accuracy of the method. Gallic acid content was quantified as 168.75 ± 1.05 mg/g methanolic extract. This developed method was simple, accurate and precise to be used as a reference standard method for determination of gallic acid content in the plant extract.
Keywords
HPLC; Standardization; quantification; Gallic acid; Acrocarpus fraxinifolius; linearity; accuracy
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