Incidence and Profiles of Antibiotic Resistance and Putative Genes of the Clostridium difficile Recovered From Fish
Rahimi, E., Nayebpour, F. (2020). Incidence and Profiles of Antibiotic Resistance and Putative Genes of the Clostridium difficile Recovered From Fish. EKB Journal Management System, 51(3), 349-356. doi: 10.21608/ejvs.2020.18342.1109
Ebrahim Rahimi; Farnaz Nayebpour. "Incidence and Profiles of Antibiotic Resistance and Putative Genes of the Clostridium difficile Recovered From Fish". EKB Journal Management System, 51, 3, 2020, 349-356. doi: 10.21608/ejvs.2020.18342.1109
Rahimi, E., Nayebpour, F. (2020). 'Incidence and Profiles of Antibiotic Resistance and Putative Genes of the Clostridium difficile Recovered From Fish', EKB Journal Management System, 51(3), pp. 349-356. doi: 10.21608/ejvs.2020.18342.1109
Rahimi, E., Nayebpour, F. Incidence and Profiles of Antibiotic Resistance and Putative Genes of the Clostridium difficile Recovered From Fish. EKB Journal Management System, 2020; 51(3): 349-356. doi: 10.21608/ejvs.2020.18342.1109

Incidence and Profiles of Antibiotic Resistance and Putative Genes of the Clostridium difficile Recovered From Fish

Egyptian Journal of Veterinary Sciences
Article 7, Volume 51, Issue 3, September 2020, Page 349-356  XML PDF (1.17 MB)
Document Type: Original Article
DOI: 10.21608/ejvs.2020.18342.1109
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Authors
Ebrahim Rahimi email 1; Farnaz Nayebpourorcid 2
1Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
2Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Abstract
Clostridium difficile (C. difficile) is a toxigenic bacterium with emergence of antibiotic resistance accountable for incidence of food poisoning. The purpose of this survey was to examine the antibiotic resistance profile and incidence of toxigenic genes amid the C. difficile bacteria recovered from dissimilar varieties of fish samples. One-hundred and eighty-four fish samples were obtained and examined by culture technique. C. difficile isolates were confirmed another time using the polymerase chain reaction. PCR and disk diffusion techniques were applied for detection of putative genes and phenotypic profile of resistance. Eleven out of 184 (5.97%) fish samples harbored C. difficile. Common carp (17.50%) had the uppermost incidence of C. difficile, though Scomberomorus guttatus (2.50%) had the lowermost. There were no positive results for Scomberomorus commerson and barracuda fish samples. TcdA (45.45%) was the most generally perceived toxigenic genes, though tcdC (18.18%) was the less frequently. There were no perceived cdtA and ctdB toxigenic genes. C. difficile bacteria displayed the uppermost incidence of resistance toward amoxicillin (63.60%), ampicillin (54.54%), moxifloxacin (54.54%) and piperacillin (54.54%). C. difficile bacteria displayed the uppermost incidence of susceptibility toward meropenem (90.90%%), vancomycin (90.90%) and metronidazole (72.72%). Common carp, rainbow trout and Scomberomorus guttatus may be reservoirs of C. difficile bacteria. Boost incidence of toxigenic and resistant C. difficile pose an imperative health threating issue rendering the consumption of raw fish samples.
Keywords
Clostridium difficile; Toxigenic genes; Antibiotic resistance; Fish
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