Prognostic Significance of Telomere Length in newly diagnosed Chronic Lymphocytic Leukemia patients: measured By FlowFISH technique | ||||
| SECI Oncology Journal | ||||
| Article 1, Volume 5, Issue 3, July 2017, Page 24-34 PDF (1 MB) | ||||
| DOI: 10.21608/secioj.2017.10551 | ||||
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| Abstract | ||||
| Background: Telomeres are specialized nucleoprotein structures present at the ends of human chromosomes, and are constituted of a tandem of repeats TTAGGG. Telomere length measurement has been assessed by many previous studies In B-Chronic Lymphocytic Leukemia patients (B-CLL). Telomere shortening was found to correlate with disease progression. The aim of our study was to measure Telomere Length (TL) by Florescence In-Situ Hybridization coupled with Flow-cytometer (Flow-FISH) technique in newly diagnosed B-CLL patients. Explore its prognostic significance, through determination of the relationship between TL; with disease stage, other prognostic marker such as CD38 & ZAP-70 expression and patient response to treatment. Methods: This study included 61 B-CLL patients in chronic phase and 67 normal healthy controls. Telomere Length (TL) was assessed in Lymphocytes by Flow-FISH technique. Results: This work revealed a significant reduction of the Relative Telomere Length (RTL) in B-CLL patients than normal healthy control. RTL was significantly negatively correlated with CD38 and ZAP70 expression measured at patient diagnosis (p= 0.09& 0.017 respectively). Patients follow up was done by BMA after six months. We observed that patients who had higher TL, showed a better response to treatment (p> 0.001). Conclusion: Telomere length may have a prognostic relevance in B-CLL cases. | ||||
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