Potentiation of antifibrotic activity of Atacand using grape seed extract (Gervital) in male albino rats | ||||
Journal of Bioscience and Applied Research | ||||
Article 1, Volume 1, Issue 1, March 2015, Page 1-9 PDF (5.09 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jbaar.2015.105944 | ||||
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Authors | ||||
Faten Abdel- Ghaffar* 1; Ibrahim A. El-Elaimy1; Mona EL. Ghreeb1; Azza M. Mohamed2 | ||||
1Department of Zoology, Faculty of Science, Menoufia University, Shebin El- Kom, Egypt | ||||
2Department of Therapeutic Chemistry, National Research Center, Dokki. Egypt | ||||
Abstract | ||||
Carbon tetrachloride (CCl4) is a known potent hepatotoxic agent. The present investigation clarified the ameliorative effects of Atacand and Gervital on CCl hepatotoxicity. 24 male albino rats were divided into 4 groups, 6 rats each. Group I, served as normal control. Group II, animals were injected subcutaneously with CCl (1ml/kg b.w.) twice a week for 90 days. Group III, rats were injected with CCl4, as Group II, then treated orally with Atacand (8mg/kg b.w.), daily, for 30 days. rats were injected with the same dose of CCl then treated with Atacand (8mg/kg b.w.) along with Gervital (100mg/kg b.w.), orally, daily for 30 days. treatment showed a marked deterioration of liver function as a high increase in the levels of serum AST and ALT (153.8% and 157.8%, respectively). Also, a significant decrease in liver antioxidant machinery was represented by reduced glutathione (GSH, 68.1%), glutathione transferase (GST, 69.9%), and glutathione reductase (GR, 65.65%). On the other side, the levels of liver protein carbonyl groups (PCO) and lipid peroxidation (LPO) were elevated 439.6% and 258.7%, respectively, compared to their control groups. These results indicated the damage as a result of CCl4-induced liver fibrosis. Both treatments with Atacand or Atacand + Gervital showed a significant ameliorative effect for both liver and serum CCl4-induced alterations. The current study recorded the treatment with Gervital along with Atacand antifibrotic effectiveness than that of Atacand Gervital acted as a potentiator for the antifibrotic activity of Atacand. | ||||
Keywords | ||||
Atacand; Carbon tetrachloride; Gervital; Liver function; Oxidative stress | ||||
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