Hemoglobin A2 Cut off Values in Egyptian Cohort as a marker of β -Thalassemia carriers.
Kablan, N., El Hamshary, M., Nasr, G., Hamza, M., Salam, T., Hemeida, A. (2015). Hemoglobin A2 Cut off Values in Egyptian Cohort as a marker of β -Thalassemia carriers.. EKB Journal Management System, 1(4), 154-159. doi: 10.21608/jbaar.2015.106021
Noura M. Kablan; Manal O. El Hamshary; Ghada M. Nasr; Mohamed T. Hamza; Tarif H. Salam; Alaa A. Hemeida. "Hemoglobin A2 Cut off Values in Egyptian Cohort as a marker of β -Thalassemia carriers.". EKB Journal Management System, 1, 4, 2015, 154-159. doi: 10.21608/jbaar.2015.106021
Kablan, N., El Hamshary, M., Nasr, G., Hamza, M., Salam, T., Hemeida, A. (2015). 'Hemoglobin A2 Cut off Values in Egyptian Cohort as a marker of β -Thalassemia carriers.', EKB Journal Management System, 1(4), pp. 154-159. doi: 10.21608/jbaar.2015.106021
Kablan, N., El Hamshary, M., Nasr, G., Hamza, M., Salam, T., Hemeida, A. Hemoglobin A2 Cut off Values in Egyptian Cohort as a marker of β -Thalassemia carriers.. EKB Journal Management System, 2015; 1(4): 154-159. doi: 10.21608/jbaar.2015.106021

Hemoglobin A2 Cut off Values in Egyptian Cohort as a marker of β -Thalassemia carriers.

Journal of Bioscience and Applied Research
Article 2, Volume 1, Issue 4, October 2015, Page 154-159  XML PDF (226.07 K)
Document Type: Original Article
DOI: 10.21608/jbaar.2015.106021
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Authors
Noura M. Kablan1; Manal O. El Hamshary1; Ghada M. Nasr* 1; Mohamed T. Hamza2; Tarif H. Salam2; Alaa A. Hemeida3
1Department of Molecular Diagnostics and Therapeutics, Genetic Engineering and Biotechnology Research Institute, University of Sadat City.
2Department of Clinical pathology, Faculty of Medicine, Ain Shams University
3Department of Bioinformatics, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt
Abstract
Beta thalassemias (β-thalassemias) are a group of inherited blood disorders caused by reduced or absent synthesis of beta chains of hemoglobin resulting in variable phenotypes ranging from severe anemia to clinically asymptomatic individuals. The total annual incidence of symptomatic individuals is estimated 1 in 100.000 throughout the world. HbA2 determination plays a key role in screening programs for β-thalassemia since a small increase in this fraction is the most important marker of β-thalassemia heterozygous carriers. This study aims to detect the cut off value of Hb A2 in Egyptian individuals with β-thalassemia and microcytic hypochromic anemia, using automated HPLC. The study was carried out on 112 male and female cases. Their ages ranged from 2 years to 35 years. They were 28 normal cases, 20 cases diagnosed as microcytic hypochromic anemia, 52 cases as β-thalassemia trait and 12 cases as β-thalassemia intermedia. Informed consents were obtained from their care givers. CBC was done to all groups and HbA2 levels were measured using HPLC Bio- Rad D-10 dual program (β-thal short program) for identification of types of Hb and determining the cut off value of Hb A2. The Hb A2 cut off value in microcytic hypochromic anemia cases was >2.2%, in β-thalassemia intermedia cases was >3.2% and in β-thalassemia trait cases was >3.6% which showed the best cut off value, and hence the cut off value of Hb A2 could be used as a factor that plays a key role in screening programs for β- thalassemia carriers.
Keywords
β-thalassmia carriers; HbA2 cut off
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