Gene polymorphism of Il-18 as a pro-inflammatory mediator and susceptibility of diabetes type 1in Egyptian children.
Ali, Y., El-Gahel, H., Abdel-Hakem, N., El-Hefnawy, M., Ebeid, M. (2018). Gene polymorphism of Il-18 as a pro-inflammatory mediator and susceptibility of diabetes type 1in Egyptian children.. EKB Journal Management System, 4(2), 20-29. doi: 10.21608/rjab.2018.106794
Yasser B.M. Ali; Hend El-Sayed El-Gahel; Nehal E. Abdel-Hakem; Mohamed Hesham El-Hefnawy; Mohammed Elshahat Ebeid. "Gene polymorphism of Il-18 as a pro-inflammatory mediator and susceptibility of diabetes type 1in Egyptian children.". EKB Journal Management System, 4, 2, 2018, 20-29. doi: 10.21608/rjab.2018.106794
Ali, Y., El-Gahel, H., Abdel-Hakem, N., El-Hefnawy, M., Ebeid, M. (2018). 'Gene polymorphism of Il-18 as a pro-inflammatory mediator and susceptibility of diabetes type 1in Egyptian children.', EKB Journal Management System, 4(2), pp. 20-29. doi: 10.21608/rjab.2018.106794
Ali, Y., El-Gahel, H., Abdel-Hakem, N., El-Hefnawy, M., Ebeid, M. Gene polymorphism of Il-18 as a pro-inflammatory mediator and susceptibility of diabetes type 1in Egyptian children.. EKB Journal Management System, 2018; 4(2): 20-29. doi: 10.21608/rjab.2018.106794

Gene polymorphism of Il-18 as a pro-inflammatory mediator and susceptibility of diabetes type 1in Egyptian children.

Research Journal of Applied Biotechnology
Article 3, Volume 4, Issue 2, November 2018, Page 20-29  XML PDF (865.81 K)
Document Type: Original Article
DOI: 10.21608/rjab.2018.106794
View on SCiNiTO View on SCiNiTO
Authors
Yasser B.M. Ali email 1; Hend El-Sayed El-Gahel1; Nehal E. Abdel-Hakem1; Mohamed Hesham El-Hefnawy2; Mohammed Elshahat Ebeid1
1Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt.
2National Institute of Diabetes and Endocrinology (NIDE), Cairo University, Egypt
Abstract
Type 1 diabetes (T1D) is a chronic immune-mediated disease and is the most common autoimmune disorder in childhoodbut the disease may become manifest at any age. It is characterized by selective loss of insulin-producing β-cells in the pancreatic islets in genetically susceptible subjects.Interleukin- 18 (IL-18) is a proinflammatory cytokinethat was originally identified as an interferon-γ inducing factor.It plays a critical role in chronic inflammation, in autoimmune diseases, and in a number of infectious diseases. Functional variants of IL18 gene has been reported as associated with T1D.the purpose of this was to investigate the role of IL-18 gene polymorphism (-607 A/C)in the prediction of T1D in the Egyptian children. One hundred and four patients with T1D and 116 healthy controls, IL- 18 promoter genotyping at -607 (rs1946518) position was analyzed with sequence-specific PCR(PCR/SSP).Therewas no significant association between polymorphisms in SNPs of IL-18 (rs1946518A/C) and T1D risk in Egyptian population,However, the results suggest an association of genotypes with age at onset of T1D.Our preliminary data suggested that polymorphisms in the IL-18 gene may not contribute to T1D susceptibility. To the best of our knowledge, this study is the first one that deals with IL-18 gene polymorphism in T1D patients in Egypt. Although, further studies with large sample size should be conducted to validate these results in Egyptian population.
Keywords
T1D; IL-18; polymorphism; PCR/SSP
Statistics
Article View: 154
PDF Download: 307