Effect of L-arginine on methotrexate induced hepatotoxicity in albino rats.
Abdel-Mawla, A., Hassan, S., Abd Al-Haleem, E., ElHangoor, S. (2016). Effect of L-arginine on methotrexate induced hepatotoxicity in albino rats.. EKB Journal Management System, 2(2), 88-99. doi: 10.21608/jbaar.2016.106909
Ashour A - S Abdel-Mawla; Safia M Hassan; Ekram N Abd Al-Haleem; Safeyah Z ElHangoor. "Effect of L-arginine on methotrexate induced hepatotoxicity in albino rats.". EKB Journal Management System, 2, 2, 2016, 88-99. doi: 10.21608/jbaar.2016.106909
Abdel-Mawla, A., Hassan, S., Abd Al-Haleem, E., ElHangoor, S. (2016). 'Effect of L-arginine on methotrexate induced hepatotoxicity in albino rats.', EKB Journal Management System, 2(2), pp. 88-99. doi: 10.21608/jbaar.2016.106909
Abdel-Mawla, A., Hassan, S., Abd Al-Haleem, E., ElHangoor, S. Effect of L-arginine on methotrexate induced hepatotoxicity in albino rats.. EKB Journal Management System, 2016; 2(2): 88-99. doi: 10.21608/jbaar.2016.106909

Effect of L-arginine on methotrexate induced hepatotoxicity in albino rats.

Journal of Bioscience and Applied Research
Article 2, Volume 2, Issue 2, February 2016, Page 88-99  XML PDF (1.34 MB)
Document Type: Original Article
DOI: 10.21608/jbaar.2016.106909
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Authors
Ashour A - S Abdel-Mawla1; Safia M Hassan* 1; Ekram N Abd Al-Haleem2; Safeyah Z ElHangoor2
1Histochemistry and Cell Biology Department, Medical Research Institute Alexandria University
2Pharmacology and Toxicology Department Faculty of Pharmacy (Girls), Al- Azhar University
Abstract
Methotrexate (MTX) is commonly used in the treatment of many different types of cancer and inflammatory diseases. Its cytotoxic nature also lends a substantial risk of life-threatening side effects. L-arginine is beneficial in the treatment of hepatic injury, hepatic cirrhosis and fatty liver degeneration. The present work aims to study the effect of L-arginine on hepatotoxicity of methotrexate in albino rats. Five groups of albino rats were used. Group I: control. Group II: rats were administered (MTX) in a daily oral dose of 0.45 mg/kg, for 28 days. Group III: rats were administered L-arginine in a daily oral dose of 300 mg/kg, for 28 days. Group IV: rats were received L- arginine 2 hrs before (MTX). Group V: rats were received L-arginine 2 hrs after (MTX). The results revealed different histopathological changes in liver of MTX-treated rats such as focal areas of necrosis and increased numbers of activated Kupffer cells, an apparent increase in the amount of collagen fibers and strong immunoreactive expression of α- SMA. Biochemical results revealed a significant increase in the serum levels of ALT, AST, bilirubin and decreasing the level of antioxidant enzymes. L-agrinine minimized the hepatotoxicity of MTX by decreasing the level of ALT, AST and bilirubin, MDA and increasing the antioxidant enzymes. It is concluded that L-arginine protects liver from hepatotoxicity of methotrexate and this due to its antioxidant activity.
Keywords
Liver; Methotrexate; L-arginine; α- SMA; ALT; AST; bilirubin; SOD; GSH and MDA
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