Evaluation of caspase 9 expression in induced hamster buccal pouch carcinoma | ||||
| Al-Azhar Journal of Dental Science | ||||
| Article 2, Volume 20, Issue 2, April 2017, Page 105-111 PDF (1.35 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ajdsm.2017.107497 | ||||
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| Abstract | ||||
| Theaimofthecurrentstudywastoevaluatethe expressionofcaspase9asanapoptoticmarkerin7, 12- Dimethylbenz(a)anthracene (DMBA)inducedhamster buccalpouch (s)(HBP(s)) carcinoma.Materialand methods:Theexperimentalanimalsusedinthecurrentstudywerefifty-fivegoldenSyrianhamstersweighting 80-129g,andobtained fromthe animalhouse,CairoUniversity.The experimentalanimals weredividedinto two main groups:Group A (normalgroup) 5hamsters weretaken, not treated and served as control. Group B:Theremaininganimals(50hamsters)weretreatedwiththesameprocedures:HBPswerepaintedfor18 weekswithDMBA,3 timesaweek.Then,a directlesionalinjectionby differentkindsoftreatment:gold nanoparticles (AuNPs) (groupB2),AuNPs+laser (groupB3)cisplatin(groupB4),AuNPsconjugatedwith cisplatin and laser irradiation (group B5), were subsequentlyemployed. After that, various investigation: hematoxylinandeosin(H&E)stainandimmunohistochemicalstainingutilizingantibodyagainstcaspase9 weredone.Results:Grossobservationrevealedthatthereis avariationinthereductionofthedistribution andsizeof tumorsbetweenthe treatedgroups.Immunohistochemicalresultsrevealed thatcaspase 9expression hasawidevariability intheareapercentagedistributionthrough-outthetreatedgroups.Conclusions: AuNPsinconjunctionwithcisplatinandvisible laserirradiationhavepositiveeffectontumor ablation. Moreover, intra-tumoral application of AuNPs in conjunction with cisplatin and visible laser irradiation is considered as a promising therapeutic agent in the regression of HBP DMBA induced carcinoma. | ||||
| Keywords | ||||
| Keywords: Squamous cell carcinoma; Apoptosis; Cisplatin; Gold nanoparticles | ||||
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