Electron microscopic evaluation of cisplatin conjugated nanogold and laser irradiation on hamster buccal pouch carcinoma
(2017). Electron microscopic evaluation of cisplatin conjugated nanogold and laser irradiation on hamster buccal pouch carcinoma. EKB Journal Management System, 20(2), 113-118. doi: 10.21608/ajdsm.2017.107498
. "Electron microscopic evaluation of cisplatin conjugated nanogold and laser irradiation on hamster buccal pouch carcinoma". EKB Journal Management System, 20, 2, 2017, 113-118. doi: 10.21608/ajdsm.2017.107498
(2017). 'Electron microscopic evaluation of cisplatin conjugated nanogold and laser irradiation on hamster buccal pouch carcinoma', EKB Journal Management System, 20(2), pp. 113-118. doi: 10.21608/ajdsm.2017.107498
Electron microscopic evaluation of cisplatin conjugated nanogold and laser irradiation on hamster buccal pouch carcinoma. EKB Journal Management System, 2017; 20(2): 113-118. doi: 10.21608/ajdsm.2017.107498

Electron microscopic evaluation of cisplatin conjugated nanogold and laser irradiation on hamster buccal pouch carcinoma

Al-Azhar Journal of Dental Science
Article 3, Volume 20, Issue 2, April 2017, Page 113-118  XML PDF (992.65 K)
Document Type: Original Article
DOI: 10.21608/ajdsm.2017.107498
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Abstract
Theaim of thecurrentstudywas toevaluate theultrastructural illustrationofapoptosis inducedbygold nanoparticles (AuNPs)conjugatedwithcisplatinand laser irradiationon7,12- Dimethylbenz(a)anthracene (DMBA) inducedhamsterbuccalpouches{HBP(s)}carcinoma.Materialand methods:Theexperimentalanimalsusedin the currentstudywerefifty fivegoldensyrianhamstersweighting80-129g,andobtainedfrom theanimalhouse,Cairo University.Theexperimentalanimalsweredividedintotwomaingroups:GroupA(normalgroup)5hamsterswere taken,nottreatedandservedascontrol.GroupB: Theremaining animals (50hamsters)weretreatedwith thesame procedure:HBPswerepainted for18 weekswith DMBA, 3 timesaweekon alternativedays,then the hamsters were randomly dividedintothefollowing fiveequalsubgroups(10 hamsters)ineachone.GroupB1( DMBAtreated group), groupB2(DMBA-AuNPstreatedgroup), group B3(DMBA-AuNPs-laser radiation treatedgroup), GroupB4(DMBA- cisplatin-laser radiationtreatedgroup)and groupB5(DMBA-AuNPsconjugatedwith cisplatin-laserradiationtreatedgroup). Aftertermination to the treatment, freshtissuesfromHBPweretrimmedintosmallpiecesinorderto beprocessedandexaminedby transmissionelectronmicroscopy(TEM).Results:comparedto resultsof othergroups, DMBA inducedcarcinoma ingroup B animals.AuNPsconjugatedwithcisplatinand laser irradiationgroupshowedsignsofcellular apoptosis ranging from shrinkageofthenuclei,degenerationofthenuclearmembrane to totalcellautolysis. Theother remaninig groupsdidn`tshowanysignsofapoptosis.Conclusions: AuNPs inconjunction with cisplatinandlaserirradiationillustratedvarious signsofcancercellapoptosiswith TEM imaging.Intra-tumoral applicationofAuNPs inconjunctionwithcisplatinandvisiblelaser irradiationwasconsideredasapromising therapeutic agent inregression ofHBP DMBAinduced carcinoma.
 
Keywords
Keywords: Squamous cell carcinoma; Apoptosis; Cisplatin; Gold nanoparticles
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