Influence of Epidermal Growth Factor with Cysteamine on in -Vitro Buffalo Embryo Development | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Article 3, Volume 47, Issue 1, June 2016, Page 27-39 PDF (378.13 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2016.1080 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Al-shimaa El-Naby1; Karima Ghoneimy M.   2; Tarek Scholkamy3; Youssef Youssef4; Gamal Sosa5; Mahmoud Abou El-Roos1
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| 1Faculty of Veterinary Medicine, Benha University | ||||
| 2prof Dr at National Research Center | ||||
| 3Animal Reproduction Research Institute | ||||
| 4National Research Center, Egypt | ||||
| 5Faculty of Veterinary Medicine, Benha University, Egypt. | ||||
| Abstract | ||||
| FOR IMPROVING embryo development in buffalo, two experiments were conducted. The first one was carried out to evaluate the different concentrations (0, 5, 25, 50 ng/ml) of epidermal growth factors (EGF) on developmental competence of buffalo oocytes. The selected oocytes were cultured in the four concentrations of EGF. The embryo cleavage rate was significantly higher in oocytes exposed to 5, 25, 50 ng/ml EGF than control. There were no significant difference in four groups (0, 5, 25, 50 ng/ml EGF) in the rate of morula. But, better cleavage and blastocyst rates were observed at 5 ng/ml EGF. In the second experiment, the additive effect of 5 ng/ml EGF with 50 µM cysteamine on maturation and embryo development was studied. Oocytes were collected, matured and cultured in three groups. In the first group, media supplemented with 5 ng/ml EGF + 50 µM cysteamine combination. In the second group, media supplemented with EGF. The third group was supplemented with cysteamine. There was a significant increase in cleavage rate in combination group than EGF (P< 0.05) and cysteamine (P< 0.01) groups. But there was no significant difference in cleavage rate between EGF and cysteamine. The morula percentage was nearly similar in the three groups. But blastocyst rate was significantly (P<0.05) higher in combination group than cysteamine. Thus better cleavage and blastocyst rate were observed at combination group. It is concluded that, the addition of 5 ng/ml EGF in buffalo oocytes during in vitro culture was the best concentration for embryo developmental competence. Higher cleavage and blastocyst rate were achieved by combination of epidermal growth factor and antioxidant. | ||||
| Keywords | ||||
| Buffalo oocytes; EGF; Cysteamine; Embryo development; culture media | ||||
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