Single Nucleotide Polymorphisms of GnRHR Gene and Its Relationship with Reproductive Performance in Egyptian Buffaloes
Sosa, A., Ghoneimy M., K., Eldebaky, H., Kandiel, M., Abou El-Roos, M., Nawito, M. (2016). Single Nucleotide Polymorphisms of GnRHR Gene and Its Relationship with Reproductive Performance in Egyptian Buffaloes. EKB Journal Management System, 47(1), 41-50. doi: 10.21608/ejvs.2016.1081
Ahmed Sosa; Karima Ghoneimy M.; Hazem Eldebaky; Mohamed Kandiel; Mahmoud Abou El-Roos; Mahmoud F. Nawito. "Single Nucleotide Polymorphisms of GnRHR Gene and Its Relationship with Reproductive Performance in Egyptian Buffaloes". EKB Journal Management System, 47, 1, 2016, 41-50. doi: 10.21608/ejvs.2016.1081
Sosa, A., Ghoneimy M., K., Eldebaky, H., Kandiel, M., Abou El-Roos, M., Nawito, M. (2016). 'Single Nucleotide Polymorphisms of GnRHR Gene and Its Relationship with Reproductive Performance in Egyptian Buffaloes', EKB Journal Management System, 47(1), pp. 41-50. doi: 10.21608/ejvs.2016.1081
Sosa, A., Ghoneimy M., K., Eldebaky, H., Kandiel, M., Abou El-Roos, M., Nawito, M. Single Nucleotide Polymorphisms of GnRHR Gene and Its Relationship with Reproductive Performance in Egyptian Buffaloes. EKB Journal Management System, 2016; 47(1): 41-50. doi: 10.21608/ejvs.2016.1081

Single Nucleotide Polymorphisms of GnRHR Gene and Its Relationship with Reproductive Performance in Egyptian Buffaloes

Egyptian Journal of Veterinary Sciences
Article 4, Volume 47, Issue 1, June 2016, Page 41-50  XML PDF (782.14 K)
Document Type: Original Article
DOI: 10.21608/ejvs.2016.1081
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Authors
Ahmed Sosaorcid 1; Karima Ghoneimy M. email orcid 2; Hazem Eldebakyorcid 1; Mohamed Kandiel3; Mahmoud Abou El-Roos4; Mahmoud F. Nawito5
1National Research Centre
2prof Dr at National Research Center
3Faculty of Veterinary Medicine, Benha University,
4Faculty of Veterinary Medicine, Benha University
5null
Abstract
THIS WORK aimed to search for single nucleotide polymorphisms of gonadotropin releasing hormone (GnRHR) gene in Egyptian buffalo which involved in endocrine control of fertility. An ultrasonographic examinations of anestrum and repeat breeders’ buffaloes wereperformed prior to blood sampling through a transrectal ultrasonography.  DNA was extracted from blood and the PCR and single-strand conformation polymorphism (SSCP) techniqueswereadopted to study of GnRHR gene polymorphism. The PCR amplified a fragment with 240-bp in size and the SSCP results showed that there isagenetic polymorphism with three different patterns (AA, BB and CC) in Egyptian buffalo. The CC genotype was associated with smooth inactive ovary,while BB genotype was associated with fertility in buffalo. Multiple sequence alignment of the three patterns sequences revealed that GnRHR had 5 single nucleotide polymorphisms including one nucleotide insertion, one nucleotide deletion and 3 nucleotide substitutions. Insertion was at position 4 in pattern III. The deletion was at position 189 in pattern III, while the 3 nucleotide substitutions were at positions 204 (T/G), 206 (T/A) and 207 (A/T). In conclusion, GnRHR gene could be used as a candidate marker for fertility in Egyptian buffaloes with its mutation is relatedto ovarian inactivity.
Keywords
Buffalo; GnRHR Gene; Nucleotide sequences; SSCP analysis
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