CHARACTERIZATION OF Β-LACTAMASE GENES IN ESCHERICIA COLI ISOLATED FROM BROILERS
Dawoud, F., Nada, H., Abdelaziz, M., El-Ikhnawey, K. (2014). CHARACTERIZATION OF Β-LACTAMASE GENES IN ESCHERICIA COLI ISOLATED FROM BROILERS. EKB Journal Management System, 12(2), 1-19. doi: 10.21608/kvmj.2014.108735
Fawzia A. Dawoud; Hisham S. Nada; Magdi I. Abdelaziz; Khalid I. El-Ikhnawey. "CHARACTERIZATION OF Β-LACTAMASE GENES IN ESCHERICIA COLI ISOLATED FROM BROILERS". EKB Journal Management System, 12, 2, 2014, 1-19. doi: 10.21608/kvmj.2014.108735
Dawoud, F., Nada, H., Abdelaziz, M., El-Ikhnawey, K. (2014). 'CHARACTERIZATION OF Β-LACTAMASE GENES IN ESCHERICIA COLI ISOLATED FROM BROILERS', EKB Journal Management System, 12(2), pp. 1-19. doi: 10.21608/kvmj.2014.108735
Dawoud, F., Nada, H., Abdelaziz, M., El-Ikhnawey, K. CHARACTERIZATION OF Β-LACTAMASE GENES IN ESCHERICIA COLI ISOLATED FROM BROILERS. EKB Journal Management System, 2014; 12(2): 1-19. doi: 10.21608/kvmj.2014.108735

CHARACTERIZATION OF Β-LACTAMASE GENES IN ESCHERICIA COLI ISOLATED FROM BROILERS

Kafrelsheikh Veterinary Medical Journal
Article 1, Volume 12, Issue 2, October 2014, Page 1-19  XML PDF (579.99 K)
Document Type: Original Article
DOI: 10.21608/kvmj.2014.108735
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Authors
Fawzia A. Dawoud* 1; Hisham S. Nada2; Magdi I. Abdelaziz3; Khalid I. El-Ikhnawey4
1Animal Health Recearch Institute, Tanta branch, Egypt
2Bacteriology,Mycology and Immunology Dep.,Fac.of vet.med. Kafreelsheikh Univ., Egypt
3Pharmacology Dep., Fac. of vet. med., Kafreelsheikh Univ., Egypt
4Animal Health Recearch Institute, Dokki, Giza, Egypt
Abstract
Due to intensive use of β-lactam drugs as therapeutic agents and
growth promoting the β-lactam resistant is developed,and it is
primarily developed by β-lactamases . therefore ,the objective of this
study was to characterize β-lactamase genes in E. coli isolated from
broilers. A total of 105 sampels isolated from broilers from Gharbia
governorates ,28 isolates were positive for E.coli (26.6%), 15 isolatas
were randomly taken and they serogrouped to 7 serotypes (O 55,
O78, O 125, O126, O 157, O119 :H6 ,and O1:H7) .All isolated strains
showed multidrug resistance phenotypes particularly against
amoxicillin, erythromycin, doxycyclin, norfloxacin, and colistin
sulphate respectively, PCR for the detection of β-lactamase genes in
isolated strains showed that , all isolates contain β-lactamase genes,
5 isolates contain bla TEM 5 (50%), 2 isolates contain bla CTX-M 2
(20%), 3 isolates contain bla OXA 3 (30%), 2 isolates contain bla
SHV 2 (20%), 1 isolates contain both bla TEM and bla OXA, 2 isolate
contain both bla TEM and bla CTX-M and 1 isolate contain three
gens bla TEM , bla SHV, and bla CTX-M .
 

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Keywords
Β-LACTAMASE; ESCHERICIA COLI; BROILERS
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