CHARACTERIZATION OF Β-LACTAMASE GENES IN ESCHERICIA COLI ISOLATED FROM BROILERS | ||||
Kafrelsheikh Veterinary Medical Journal | ||||
Article 1, Volume 12, Issue 2, October 2014, Page 1-19 PDF (579.99 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/kvmj.2014.108735 | ||||
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Authors | ||||
Fawzia A. Dawoud* 1; Hisham S. Nada2; Magdi I. Abdelaziz3; Khalid I. El-Ikhnawey4 | ||||
1Animal Health Recearch Institute, Tanta branch, Egypt | ||||
2Bacteriology,Mycology and Immunology Dep.,Fac.of vet.med. Kafreelsheikh Univ., Egypt | ||||
3Pharmacology Dep., Fac. of vet. med., Kafreelsheikh Univ., Egypt | ||||
4Animal Health Recearch Institute, Dokki, Giza, Egypt | ||||
Abstract | ||||
Due to intensive use of β-lactam drugs as therapeutic agents and growth promoting the β-lactam resistant is developed,and it is primarily developed by β-lactamases . therefore ,the objective of this study was to characterize β-lactamase genes in E. coli isolated from broilers. A total of 105 sampels isolated from broilers from Gharbia governorates ,28 isolates were positive for E.coli (26.6%), 15 isolatas were randomly taken and they serogrouped to 7 serotypes (O 55, O78, O 125, O126, O 157, O119 :H6 ,and O1:H7) .All isolated strains showed multidrug resistance phenotypes particularly against amoxicillin, erythromycin, doxycyclin, norfloxacin, and colistin sulphate respectively, PCR for the detection of β-lactamase genes in isolated strains showed that , all isolates contain β-lactamase genes, 5 isolates contain bla TEM 5 (50%), 2 isolates contain bla CTX-M 2 (20%), 3 isolates contain bla OXA 3 (30%), 2 isolates contain bla SHV 2 (20%), 1 isolates contain both bla TEM and bla OXA, 2 isolate contain both bla TEM and bla CTX-M and 1 isolate contain three gens bla TEM , bla SHV, and bla CTX-M . | ||||
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Keywords | ||||
Β-LACTAMASE; ESCHERICIA COLI; BROILERS | ||||
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