MOLECULAR CHARACTERIZATION OF NEWCASTLE DISEASE VIRUS ISOLATED FROM SAUDI ARABIA | ||||
| Kafrelsheikh Veterinary Medical Journal | ||||
| Article 15, Volume 14, Issue 1, May 2016, Page 257-267 PDF (437.12 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/kvmj.2016.108855 | ||||
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| Authors | ||||
| Mahmoud M. Ismail* 1; Abdulrahman Alankari2; Mahmoud H. A. Mohamed2; Faris Elkhayat3 | ||||
| 1Dept. of Poultry Diseases, college of Veterinary Medicine, Kafrelsheikh University, Egypt. Dept. of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia | ||||
| 2Dept. of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia | ||||
| 3Dept. of Poultry Diseases, college of Veterinary Medicine, Kafrelsheikh University, Egypt | ||||
| Abstract | ||||
| A total of 34 samples were collected from chicken and pigeon flocks suspected to have Newcastle disease. NDV virus was isolated and identified by inoculation into the allantoic cavity of Specific–pathogen- free chicken eggs followed by hemagglutination and hemagglutination inhibition test. Furthermore, these isolates were geno-typically analysed using RT-PCR with specific primers flanking a 270 bp region representing the hypervariable region of F gene and the PCR-product was sequenced and compared to the previously published sequences. In this study, the F protein of 34 Saudi NDV isolates phylogram revealed clustering with NDVs representing lineage 2,4 and 5. | ||||
| Keywords | ||||
| Newcastle disease virus; chicken and pigeon flocks | ||||
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