BASIC AND LECTIN HISTOCHEMISTRY OF THE PREGNANT AND NON PREGNANT VAGINAL MUCOSA OF SHE-CAME | ||||
| Kafrelsheikh Veterinary Medical Journal | ||||
| Article 1, Volume 2, Issue 1, March 2004, Page 1-16 PDF (531.1 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/kvmj.2004.109286 | ||||
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| Authors | ||||
| Masoud H. Fayed* ; Mohamed A Kassab | ||||
| DEPARTMENT OF ANATOMY AND HISTOLOGY, FACULTY OF VETERINARY MEDICINE, KAFER EL-SHEIKIH | ||||
| Abstract | ||||
| Traditional non-lectin staining methods as periodic acid Schiff (PAS) and Alcian blue at pH 2.5 (Ab 2.5), as will as horseradish peroxidase (HRP) conjugated Helix pomatia agglutinin (HPA) and Girffonia simplicifolia agglutinin(GSA-1)were usedtoinvestigate the pregnancy associated changes on the vaginal mucosa of She-camel. There was remarkable increase in the glycoproteins(GPs) with oxidizable vicinal diolesintheuppermostlayerof the vaginal epithelium of the pregnant She-camel. At the same time, only little increase in the GPs with carboxyl group and/or with O-sulfate ester was limited to the goblet cells of the vaginal mucosa. Significant changes could be detected in the lectin histochemistry in pregnant She-camel comparing with the non-pregnant one.Abundantamountof N-acetylgalactosaminedetected in the plasmalemma of all layers of the vaginal mucosa of She-camel especially in the mucous secreting cells. The changes of D-galactos and/or N- acetylgalactosamine were restricted to the goblet cells. | ||||
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| Keywords | ||||
| Glycoprotein; Lectin; histochemistry; Camel, Vagina | ||||
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