IDENTIFICATION, EXPRESSION AND LOCALIZATION OF THE UL45 PROTEIN OF EQUINE HERPES VIRUS TYPE 1 | ||||
Kafrelsheikh Veterinary Medical Journal | ||||
Article 3, Volume 13, Issue 1, April 2015, Page 35-55 PDF (910 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/kvmj.2015.109535 | ||||
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Authors | ||||
Samy Kasem 1; Gabr Albagory2; Ali Abdelkader3; Hideto Fukushi4; Noura Alkhalefa1; Mohamed Nayel5; Tarek Kamal6; Al-Said Dawod7 | ||||
1Department of Virology, Faculty of Veterinary Medicine, kafrelsheikh University, kafrelsheikh, 33516, Egypt | ||||
2Department of Virology, Faculty of Veterinary Medicine, Benha University, Egypt | ||||
3Department of Anatomy, Faculty of Veterinary Medicine, kafrelsheikh University, kafrelsheikh, 33516, Egypt | ||||
4Department of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan | ||||
5Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Sadat City University, Sadat City, Egypt | ||||
6Department of Biochemistry, Faculty of Veterinary Medicine, kafrelsheikh University, kafrelsheikh, 33516, Egypt | ||||
7Animal research institute, Kafrelsheikh branch, El Geish Street, Kafrelsheikh, Egyp | ||||
Abstract | ||||
UL45 was one of the tegument proteins that have been identified in both EHV-1 and EHV-4, it was recorded that ORF 15 EHV-1was homologues to UL45 (HSV). Until now, the function of UL45 protein in cell culture and animal models is poorly understood. To investigate the function of the UL45 protein, we expressed the UL45 gene in prokaryotic expression vector pGEX-6P-1 to express UL45 protein and used the resulting protein to raise guinea pig antiserum. In western blotting experiments, the antiserum directed against the N-terminal of EHV-1 UL45 protein specifically recognized a protein with molecular weight ranging from 30 to 60 kDa in the infected cells lysates. The immunofluorescence assay also showed that EHV-1 ORF15 antiserum was able to probe the UL45 protein which is localized mainly in the cytoplasm associating with the membrane of the cells infected with EHV-1 Ab4p. | ||||
Keywords | ||||
Equine herpes virus -1 (EHV-1); UL45 protein; Protein expression; Polyclonal antibody; Immunofluorescence assay | ||||
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