IMPACT OF INTERLEUKIN-28 B (rs12979860) GENE POLYMORPHISM ON INTRAFAMILIAL TRANSMISSION OF HCV IN EGYPT.
Elwan, N., Assal, F., Aboali, L., Effat, L., Sayed, K., Shalaby, S., Abdelhamid, M., Elkhouly, R. (2023). IMPACT OF INTERLEUKIN-28 B (rs12979860) GENE POLYMORPHISM ON INTRAFAMILIAL TRANSMISSION OF HCV IN EGYPT.. EKB Journal Management System, 29(1.1), 70-75. doi: 10.21608/zumj.2020.31255.1866
Nadia Elwan; Fathia Assal; Lobna Aboali; Laila Effat; Khalda Sayed; Safinaz Shalaby; Mohamad Abdelhamid; Reham Abdelkader Elkhouly. "IMPACT OF INTERLEUKIN-28 B (rs12979860) GENE POLYMORPHISM ON INTRAFAMILIAL TRANSMISSION OF HCV IN EGYPT.". EKB Journal Management System, 29, 1.1, 2023, 70-75. doi: 10.21608/zumj.2020.31255.1866
Elwan, N., Assal, F., Aboali, L., Effat, L., Sayed, K., Shalaby, S., Abdelhamid, M., Elkhouly, R. (2023). 'IMPACT OF INTERLEUKIN-28 B (rs12979860) GENE POLYMORPHISM ON INTRAFAMILIAL TRANSMISSION OF HCV IN EGYPT.', EKB Journal Management System, 29(1.1), pp. 70-75. doi: 10.21608/zumj.2020.31255.1866
Elwan, N., Assal, F., Aboali, L., Effat, L., Sayed, K., Shalaby, S., Abdelhamid, M., Elkhouly, R. IMPACT OF INTERLEUKIN-28 B (rs12979860) GENE POLYMORPHISM ON INTRAFAMILIAL TRANSMISSION OF HCV IN EGYPT.. EKB Journal Management System, 2023; 29(1.1): 70-75. doi: 10.21608/zumj.2020.31255.1866

IMPACT OF INTERLEUKIN-28 B (rs12979860) GENE POLYMORPHISM ON INTRAFAMILIAL TRANSMISSION OF HCV IN EGYPT.

Zagazig University Medical Journal
Article 10, Volume 29, Issue 1.1, January 2023, Page 70-75  XML PDF (241.97 K)
Document Type: Original Article
DOI: 10.21608/zumj.2020.31255.1866
View on SCiNiTO View on SCiNiTO
Authors
Nadia Elwan1; Fathia Assal1; Lobna Aboali1; Laila Effat2; Khalda Sayed3; Safinaz Shalaby4; Mohamad Abdelhamid5; Reham Abdelkader Elkhouly email 6
11Department of Tropical Medicine, Faculty of medicine, Tanta University, Tanta, Egypt
2National research center, Cairo,Egypt
3National research center, Cairo, Egypt
43Department of Public Health, Faculty of medicine, Tanta University, Tanta, Egypt
5National research center, cairo,Egypt
6Tropical medicine and infectious diseases, Faculty of medicine,Tanta university
Abstract
Background: Intra-familial transmission of hepatitis C virus (HCV) in Egypt was found to play a major role in the high prevalence of HCV in Egypt. A single nucleotide polymorphism (SNP) near the interleukin-28B (IL28B) (rs12979860) gene is associated with self-cure of Hepatitis C Virus (HCV). Aim of the work: To determine the significance of IL-28B (rs12979860) gene polymorphism in intra-familial transmission of HCV in Egypt, Patients and Methods: Three hundred HCV patients, 860 family members and 100 healthy subjects were studied. HCV antibodies test was done by Enzyme Linked Immunosorbent Assay (ELISA) for all family members. Real-Time Polymerase chain reaction (PCR) ascertained presence of chronic HCV. PCR and restriction enzyme analysis were used to determine the molecular structure of IL-28B (rs12979860) gene to all studied subjects. Results: The distribution of IL-28B (rs12979860) gene polymorphism in patients was 24.3%, 53.7% and 22% for C/C, C/T and T/T genotypes respectively, in infected family members was 27.5%, 54.9% and 17.6% for C/C, C/T and T/T respectively and in negative family members was 28.5%, 54.1% and 17.4% for C/C, C/T and T/T respectively. C/C genotype was significantly more frequent in non-infected family members while T/T genotype was significantly more frequent in HCV infected patients when compared with controls. Conclusions: Our study concluded that IL-28B (rs12979860) gene polymorphism has no impact on intra-familial transmission of HCV.
Keywords
Egypt; HCV; IL-28B, intra-familial
Statistics
Article View: 275
PDF Download: 92