ISOLATION AND MOLECULAR CHARACTERIZATION OF FOOT AND MOUTH DISEASE VIRUS IN KAFRELSHEIKH GOVERNORATE, EGYPT 2014
Kasem, S., Magouz, A., EL- Bagoury, G., Abdelaty, M., Zaghlol, A. (2015). ISOLATION AND MOLECULAR CHARACTERIZATION OF FOOT AND MOUTH DISEASE VIRUS IN KAFRELSHEIKH GOVERNORATE, EGYPT 2014. EKB Journal Management System, 13(2), 17-35. doi: 10.21608/kvmj.2015.109773
Samy Kasem; Asmaa Magouz; G. Fekry EL- Bagoury; Magdy Abdelaty; Abeer Zaghlol. "ISOLATION AND MOLECULAR CHARACTERIZATION OF FOOT AND MOUTH DISEASE VIRUS IN KAFRELSHEIKH GOVERNORATE, EGYPT 2014". EKB Journal Management System, 13, 2, 2015, 17-35. doi: 10.21608/kvmj.2015.109773
Kasem, S., Magouz, A., EL- Bagoury, G., Abdelaty, M., Zaghlol, A. (2015). 'ISOLATION AND MOLECULAR CHARACTERIZATION OF FOOT AND MOUTH DISEASE VIRUS IN KAFRELSHEIKH GOVERNORATE, EGYPT 2014', EKB Journal Management System, 13(2), pp. 17-35. doi: 10.21608/kvmj.2015.109773
Kasem, S., Magouz, A., EL- Bagoury, G., Abdelaty, M., Zaghlol, A. ISOLATION AND MOLECULAR CHARACTERIZATION OF FOOT AND MOUTH DISEASE VIRUS IN KAFRELSHEIKH GOVERNORATE, EGYPT 2014. EKB Journal Management System, 2015; 13(2): 17-35. doi: 10.21608/kvmj.2015.109773

ISOLATION AND MOLECULAR CHARACTERIZATION OF FOOT AND MOUTH DISEASE VIRUS IN KAFRELSHEIKH GOVERNORATE, EGYPT 2014

Kafrelsheikh Veterinary Medical Journal
Article 2, Volume 13, Issue 2, October 2015, Page 17-35  XML PDF (796.81 K)
Document Type: Original Article
DOI: 10.21608/kvmj.2015.109773
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Authors
Samy Kasem email 1; Asmaa Magouz1; G. Fekry EL- Bagoury2; Magdy Abdelaty3; Abeer Zaghlol1
1Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University
2Department of Virology, Faculty of Veterinary Medicine, Banha University
3Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt
Abstract
Foot and mouth disease virus (FMDV) is the most economically important veterinary pathogen due to its highly infectious nature and ability to cause persistent infection.In the period betweenAugust to October2014, an outbreak ofFoot and Mouth Disease(FMD) occurred in Egypt. This study was designated to isolate and detect the current strains of FMDV circulating in Egypt. Twenty sixtissue samples were collected from clinically diseased cattle and buffaloes from Kafrelsheikh province.The isolation of the causative agent wasdone by inoculation of baby mice intraperitoneally then passed to Baby Hamster Kidney-21 (BHK-21) cells. The inoculated mice showed paralysis and death, while the infected BHK-21 showed clear CPE (cell rounding and cell death). Molecular characterizations of the FMDV were doneusing universal primers followed by serotype specific primers for A, O, C and SAT2. Only serotypes SAT2 and A were detected with an overall detection rate of 53.8%.Results of phylogenetic tree of the current FMDV strains revealed that there is no significant divergence among the recent isolated strains and other Egyptian isolated strains from different localities.
Keywords
FMDV; Isolation And Molecular Characterization
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