EFFECT OF BOVINE SERUM ADDED TO MATURATION MEDIUM ON IN VITRO-MATURATION AND SOME GENES EXPRESSIONS OF BUFFALO OOCYTES
Shamiah, S., Kadoom, A., Ashour, A. (2015). EFFECT OF BOVINE SERUM ADDED TO MATURATION MEDIUM ON IN VITRO-MATURATION AND SOME GENES EXPRESSIONS OF BUFFALO OOCYTES. EKB Journal Management System, 13(2), 67-82. doi: 10.21608/kvmj.2015.109790
Sh.M. Shamiah; A. K. Kadoom; A. F. Ashour. "EFFECT OF BOVINE SERUM ADDED TO MATURATION MEDIUM ON IN VITRO-MATURATION AND SOME GENES EXPRESSIONS OF BUFFALO OOCYTES". EKB Journal Management System, 13, 2, 2015, 67-82. doi: 10.21608/kvmj.2015.109790
Shamiah, S., Kadoom, A., Ashour, A. (2015). 'EFFECT OF BOVINE SERUM ADDED TO MATURATION MEDIUM ON IN VITRO-MATURATION AND SOME GENES EXPRESSIONS OF BUFFALO OOCYTES', EKB Journal Management System, 13(2), pp. 67-82. doi: 10.21608/kvmj.2015.109790
Shamiah, S., Kadoom, A., Ashour, A. EFFECT OF BOVINE SERUM ADDED TO MATURATION MEDIUM ON IN VITRO-MATURATION AND SOME GENES EXPRESSIONS OF BUFFALO OOCYTES. EKB Journal Management System, 2015; 13(2): 67-82. doi: 10.21608/kvmj.2015.109790

EFFECT OF BOVINE SERUM ADDED TO MATURATION MEDIUM ON IN VITRO-MATURATION AND SOME GENES EXPRESSIONS OF BUFFALO OOCYTES

Kafrelsheikh Veterinary Medical Journal
Article 5, Volume 13, Issue 2, October 2015, Page 67-82  XML PDF (691.29 K)
Document Type: Original Article
DOI: 10.21608/kvmj.2015.109790
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Authors
Sh.M. Shamiah1; A. K. Kadoom* 2; A. F. Ashour2
1Animal Production Research Institute, Agriculture Research Center, Egypt
2Animal Production Research Institute, Agriculture Research Center, Egypt.
Abstract
The effect of supplementing TCM-199 culture medium with bovine sera on buffalo’s oocytes maturation in vitro and expression of  some related genes were studied. A total 1149 immature buffalo oocytes were randomly divided into three groups, G1( n=315) were cultured in medium with no serum supplement(control), G2( n=531) were cultured in medium supplemented with 10% Fetal calf serum (FCS) and G3(n= 303) were cultured in medium supplemented with 10% Estrous cow serum (ECS). After maturation, oocytes in each group were classified according to its shape and expansion into 2 subgroups, good (GO) and fair (FO) expanded oocytes. Expression of GAPDH, CX43, IGF II and β- Actin genes were studied in each subgroup. Results revealed that G3 and G2 showed high maturation rate (P
Keywords
buffalo oocytes; IVM; gene expression; type of serum
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