OCCURRENCE OF QUINOLONE RESISTANCE GENES AMONG SALMONELLA SPECIES ISOLATED FROM CHICKENS
Abo-Remela, E., Gad, W., Helmy, S., Hassan, W. (2015). OCCURRENCE OF QUINOLONE RESISTANCE GENES AMONG SALMONELLA SPECIES ISOLATED FROM CHICKENS. EKB Journal Management System, 13(2), 129-148. doi: 10.21608/kvmj.2015.109851
Etab M. Abo-Remela; Wafaa M. Gad; Salwa M. Helmy Helmy; Wafaa M. Hassan. "OCCURRENCE OF QUINOLONE RESISTANCE GENES AMONG SALMONELLA SPECIES ISOLATED FROM CHICKENS". EKB Journal Management System, 13, 2, 2015, 129-148. doi: 10.21608/kvmj.2015.109851
Abo-Remela, E., Gad, W., Helmy, S., Hassan, W. (2015). 'OCCURRENCE OF QUINOLONE RESISTANCE GENES AMONG SALMONELLA SPECIES ISOLATED FROM CHICKENS', EKB Journal Management System, 13(2), pp. 129-148. doi: 10.21608/kvmj.2015.109851
Abo-Remela, E., Gad, W., Helmy, S., Hassan, W. OCCURRENCE OF QUINOLONE RESISTANCE GENES AMONG SALMONELLA SPECIES ISOLATED FROM CHICKENS. EKB Journal Management System, 2015; 13(2): 129-148. doi: 10.21608/kvmj.2015.109851

OCCURRENCE OF QUINOLONE RESISTANCE GENES AMONG SALMONELLA SPECIES ISOLATED FROM CHICKENS

Kafrelsheikh Veterinary Medical Journal
Article 9, Volume 13, Issue 2, October 2015, Page 129-148  XML PDF (884.83 K)
Document Type: Original Article
DOI: 10.21608/kvmj.2015.109851
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Authors
Etab M. Abo-Remela* 1; Wafaa M. Gad2; Salwa M. Helmy Helmy3; Wafaa M. Hassan4
1Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Kafrelsheikh University
2Animal Health Research Institute, Dakahlia branch
3Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Kafrelsheikh University.
4Animal Health Research Institute, Dokki Lab., Giza.
Abstract
Resistance to antimicrobial agents within nontyphoidal Salmonella serotypes is considered a serious problem worldwide so this study was aimed to investigate the wide spread Plasmid-mediated quinolone resistance between different Salmonellae isolated from chickens .The antimicrobial susceptibility was applied on all isolates then PCR was applied for most resistant isolates to quinolones to detect the Plasmid-mediated quinolone resistance genes (qnrA, qnrB, qnrS). Forty one Salmonella isolates (9.1%) were obtained from 450 samples. All isolates exhibit resistance against all antimicrobial agents except amikacin in which give no resistance. All isolates showed resistance with 100% against nalidixic acid and flumequin but 4 isolates showed the lowest resistance (9.7%) to levofloxacin. Five isolates were sensitive to amoxicillin giving the lowest percentage (12.1%). Twenty two Salmonella isolates from a total of 41 isolates were examined by PCR for Plasmid-mediated quinolone resistance genes (qnrA, qnrB, and qnrS). while 19 Salmonella isolates not examined as they were less resistant to quinolones. Five isolates (5/22) (22.7%) were positive for qnrA, 2 isolates (2/22) (9%) were positive for qnrB and 4 isolates (4/22) (18%) were positive for qnrS.
Keywords
Salmonella; Plasmid-mediated resistance genes; chickens; Egypt
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