BACTERIOLOGICAL STUDIES OF CLOSTRIDIUM PERFRINGENS IN FISH | ||||
| Kafrelsheikh Veterinary Medical Journal | ||||
| Article 4, Volume 12, Issue 1, April 2014, Page 71-83 PDF (540.51 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/kvmj.2014.109859 | ||||
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| Authors | ||||
| Lamyaa M. Reda* 1; H. Mona2 | ||||
| 1Central Lab., Vet. Hospital, Faculty of Vet. Med., Zagazig Univ., Egypt | ||||
| 2Animal Health Research Institute, Zagazig, Egypt | ||||
| Abstract | ||||
| The current research was carried out on one hundred and thirteen random fish samples (56 processed and 57 unprocessed) collected from different localities in El-Sharkia Governorate, Egypt to obtain a complete picture of C. perfringens. Bacteriological and biochemical examination was done on the isolates. Nagler's test was applied on the recovered. C. perfringens isolates. The total incidence of positive samples from 56 processed fish samples were 32 isolates (57.1%) and from 57 unprocessed fish samples were 34 isolates (59.6%). The. Typing of C. perfringens isolates revealed that the incidence of toxigenic and non-toxigenic isolates were 84.8 and 15.2% respectively. Typing of toxigenic strains of C. perfringens revealed that C. perfringens type A was the most predominant one comparing with type D. Immune diffusion test showed that 17 and 11 toxins of types A and D gave identified reaction with incidences of 47.2 and 55% respectively. | ||||
| Keywords | ||||
| C. perfringens; Bacteriological and biochemical examination | ||||
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