LEPTIN GENE SNPS AND THEIR EFFECT ON MILK TRAITS IN EGYPTIAN BUFFALO (BUBALUS BUBALIS)
Abdo, S., Abu El-Magd, M., ElNahas, A., Mansour, A. (2014). LEPTIN GENE SNPS AND THEIR EFFECT ON MILK TRAITS IN EGYPTIAN BUFFALO (BUBALUS BUBALIS). EKB Journal Management System, 12(1), 97-110. doi: 10.21608/kvmj.2014.110129
Safaa ELsayed Abdo; Mohammed Abu El-Magd; Abeer Fikry ElNahas; Ali Abd El-Kader Mansour. "LEPTIN GENE SNPS AND THEIR EFFECT ON MILK TRAITS IN EGYPTIAN BUFFALO (BUBALUS BUBALIS)". EKB Journal Management System, 12, 1, 2014, 97-110. doi: 10.21608/kvmj.2014.110129
Abdo, S., Abu El-Magd, M., ElNahas, A., Mansour, A. (2014). 'LEPTIN GENE SNPS AND THEIR EFFECT ON MILK TRAITS IN EGYPTIAN BUFFALO (BUBALUS BUBALIS)', EKB Journal Management System, 12(1), pp. 97-110. doi: 10.21608/kvmj.2014.110129
Abdo, S., Abu El-Magd, M., ElNahas, A., Mansour, A. LEPTIN GENE SNPS AND THEIR EFFECT ON MILK TRAITS IN EGYPTIAN BUFFALO (BUBALUS BUBALIS). EKB Journal Management System, 2014; 12(1): 97-110. doi: 10.21608/kvmj.2014.110129

LEPTIN GENE SNPS AND THEIR EFFECT ON MILK TRAITS IN EGYPTIAN BUFFALO (BUBALUS BUBALIS)

Kafrelsheikh Veterinary Medical Journal
Article 6, Volume 12, Issue 1, April 2014, Page 97-110  XML PDF (468.01 K)
Document Type: Original Article
DOI: 10.21608/kvmj.2014.110129
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Authors
Safaa ELsayed Abdo* 1; Mohammed Abu El-Magdorcid 2; Abeer Fikry ElNahas3; Ali Abd El-Kader Mansour2
1Department of Animal Wealth Development, Faculty of Veterinary Medicine, Kafrelsheikh University
2Department of Anatomy& Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University
3Department of Animal Husbandry and Wealth development, Faculty of Veterinary Medicine, Alexandria University
Abstract
Leptin plays an important role in the regulation of development of the mammary gland and its polymorphisms were shown to be associated with milk quantity and quality in cattle. However, no SNP was detected and no association studies was conducted on leptin gene in Egyptian buffalo. Therefore, the objective of this study was to detect leptin gene polymorphisms and to determine associations between these polymorphisms and milk traits in Egyptian water buffalo. A PCR product of 400bp, including intron 2 of leptin gene was amplified using PCR and subsequently, subjected to sequence analysis to identify its different allelic patterns. Two novel SNPs,G247T at nucleotide number 963 and T282G at nucleotide number 998, were detected in LEP intron 2 in Egyptian buffaloes. The associations analysis revealed no significance association between these SNPs and milk traits(P>0.05) in all examined animals. Consequently, our results indicated that the LEPG247T and T282GSNPs does not influence milk yield or milk composition in Egyptian buffalo and these SNPs could not be used in a marker assisted selection program to improve milk traits in Egyptian buffalo
Keywords
Leptin; Egyptian buffalo; PCR; sequencing; SNPs; milk traits
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