MOLECULAR CHARACTERIZATION OF ANTIBIOTIC RESISTANCE IN GRAM – NEGATIVE BACTERIA ISOLATED FROM DAIRY PRODUCTS
M.I., A., S.N., H., A.E., A. (2014). MOLECULAR CHARACTERIZATION OF ANTIBIOTIC RESISTANCE IN GRAM – NEGATIVE BACTERIA ISOLATED FROM DAIRY PRODUCTS. EKB Journal Management System, 12(2), 39-61. doi: 10.21608/kvmj.2014.110142
Amany M.I.; Hisham S.N.; Ahmed A.E.. "MOLECULAR CHARACTERIZATION OF ANTIBIOTIC RESISTANCE IN GRAM – NEGATIVE BACTERIA ISOLATED FROM DAIRY PRODUCTS". EKB Journal Management System, 12, 2, 2014, 39-61. doi: 10.21608/kvmj.2014.110142
M.I., A., S.N., H., A.E., A. (2014). 'MOLECULAR CHARACTERIZATION OF ANTIBIOTIC RESISTANCE IN GRAM – NEGATIVE BACTERIA ISOLATED FROM DAIRY PRODUCTS', EKB Journal Management System, 12(2), pp. 39-61. doi: 10.21608/kvmj.2014.110142
M.I., A., S.N., H., A.E., A. MOLECULAR CHARACTERIZATION OF ANTIBIOTIC RESISTANCE IN GRAM – NEGATIVE BACTERIA ISOLATED FROM DAIRY PRODUCTS. EKB Journal Management System, 2014; 12(2): 39-61. doi: 10.21608/kvmj.2014.110142

MOLECULAR CHARACTERIZATION OF ANTIBIOTIC RESISTANCE IN GRAM – NEGATIVE BACTERIA ISOLATED FROM DAIRY PRODUCTS

Kafrelsheikh Veterinary Medical Journal
Article 3, Volume 12, Issue 2, October 2014, Page 39-61  XML PDF (741.48 K)
Document Type: Original Article
DOI: 10.21608/kvmj.2014.110142
View on SCiNiTO View on SCiNiTO
Authors
Amany M.I.* 1; Hisham S.N.1; Ahmed A.E.2
1Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University
2Animal Health Research Institute, Mansoura Laboratory
Abstract
The present work was carried out on 127 dairy products samples (62 buffalo milk, 46 cow milk, 16 kariesh cheeses and 3 yoghurt) were obtained from retail markets in Dakahlia governorate. (214) isolates were assayed for antimicrobial susceptibility, the presence of integrons and antimicrobial resistance genes. 152 out of 214 (71.02%) Gram – negative bacterial isolates showed multidrug resistance phenotypes for two or more of the following antimicrobial agents: ampicillin, streptomycin, gentamicin, tetracycline, trimethoprim/ sulfamethoxazole, nalidixic acid, ciprofloxacin, amoxicillin - clavulanic acid , chloramphenicol and cefotaxime . PCR screening for integrons showed that  eight (3.73%) isolates of (Enterobacter aerogens, Klebsiella pneumoniae, Klebsiella oxytoca, Citrobacter diversus, Citrobacter freundii, Proteus vulgaris, Escherichia coli and Serratia marsescens) were positive for class 1 integron and all isolates were negative for class 2 integron .β-lactamase resistance gene blaTEM was identified in 6 (2.80%) isolates of (K. oxytoca , E. coli , S. liquefaciens ,E. cloacae, K. pneumoniae and C. diversus). blaCTX-M  was  identified in 3 (1.40%) isolates of
(E. coli , K. oxytoca, and S. marsescens).All isolates were negative for blaCMY.
These results highlighted the role of antimicrobial use in dairy animals and development of transferable gene in bacteria in dairy animals from which such genes can be disseminated by horizontal gene transfer to other bacteria and reach human pathogens.
Keywords
Integrons; β-lactamases; blaTEM. blaCTX-M; blaCMY
Statistics
Article View: 158
PDF Download: 193