MOLECULAR CHARACTERIZATION OF EQUINE HERPES VIRUS -1 (EHV-1) IN EGYPT | ||||
| Kafrelsheikh Veterinary Medical Journal | ||||
| Article 4, Volume 16, Issue 2, October 2018, Page 61-73 PDF (393.9 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/kvmj.2018.110208 | ||||
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| Authors | ||||
| Noura .F. Alkhalefa* 1; Ismail .I. Elkon2; Neven .A. Tolba3; Effat .L. Elsayed4 | ||||
| 1Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt. | ||||
| 2Department of Gynecology &Artificial Insemination, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt | ||||
| 3Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt | ||||
| 4Rinder pest like disease department, Veterinary Serum & Vaccine Research Institute –Abasia –Cairo. | ||||
| Abstract | ||||
| Equine Herpes Virus -1 is one of the most viruses affecting equide family(Horse, Donkey &Mule) causing severe economic losses to horse industry all over the world, due to respiratory manifestations, abortion of pregnant mares ,and mayeloencephalopathy. Little reports have been made for investigation the prevalence and existence of equine herpes viruses (EHVs) in Egypt. In the present study, seventy clinical samples (6 placentas & 64 nasal swabs) collected from different governorate in Egypt. Virus isolation by inoculating tissue suspensions into MDBK cells revealed characteristic CPE for EHV after the fourth passage within 7 days from inoculation in the form of aggregation of the cells together, rounding, and finally cell detachment. Serological confirmation of the isolated sample by FAT revealed intracytoplasmic greenish yellow fluorescence. DNA extraction and PCR of suspected samples existed Successful amplification of 700 bp of UL45 gene of EHV-1 in 8 out of 70 examined samples. | ||||
| Keywords | ||||
| Equine; Egypt; EHV-1; Virus isolation; FAT and PCR | ||||
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