Effect of Platelet-rich Plasma on MMP-13, ARE and TGF β1 in MIA-Induced Osteoarthritis in Rats
M. Halfaya, F., G.H., R., U., H., O.M., A., W., A. (2020). Effect of Platelet-rich Plasma on MMP-13, ARE and TGF β1 in MIA-Induced Osteoarthritis in Rats. EKB Journal Management System, 27(2), 109-118. doi: 10.21608/jvmr.2020.34762.1019
Fatma M. Halfaya; Ragab G.H.; Hagag U.; Ahmed O.M.; Abou Elkheir W.. "Effect of Platelet-rich Plasma on MMP-13, ARE and TGF β1 in MIA-Induced Osteoarthritis in Rats". EKB Journal Management System, 27, 2, 2020, 109-118. doi: 10.21608/jvmr.2020.34762.1019
M. Halfaya, F., G.H., R., U., H., O.M., A., W., A. (2020). 'Effect of Platelet-rich Plasma on MMP-13, ARE and TGF β1 in MIA-Induced Osteoarthritis in Rats', EKB Journal Management System, 27(2), pp. 109-118. doi: 10.21608/jvmr.2020.34762.1019
M. Halfaya, F., G.H., R., U., H., O.M., A., W., A. Effect of Platelet-rich Plasma on MMP-13, ARE and TGF β1 in MIA-Induced Osteoarthritis in Rats. EKB Journal Management System, 2020; 27(2): 109-118. doi: 10.21608/jvmr.2020.34762.1019

Effect of Platelet-rich Plasma on MMP-13, ARE and TGF β1 in MIA-Induced Osteoarthritis in Rats

Journal of Veterinary Medical Research
Article 1, Volume 27, Issue 2, December 2020, Page 109-118  XML PDF (888.89 K)
Document Type: Original Article
DOI: 10.21608/jvmr.2020.34762.1019
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Authors
Fatma M. Halfaya email 1; Ragab G.H.1; Hagag U.1; Ahmed O.M.2; Abou Elkheir W.3
1Surgery, Anesthesiology and Radiology Department, Faculty of Veterinary Medicine, Beni Suef University, Egypt
2Physiology Division, Zoology Department, Faculty of Science, Egypt
3Department of Immunology, Military Medical Academy, Cairo, Egypt
Abstract
Ankle osteoarthritis (OA) is an inflammatory deterioration chronic disease; yet, OA pathogenesis is obscure. There is no absolute cure from OA and the present pharmacological medication options are constrained and associated with adverse aspect effects. Clinically, Platelet-rich plasma (PRP) is usually used to cure OA. The present study was designed to evaluate the role of PRP in the treatment of experimentally Monosodium iodoacetate (MIA)-induced ankle osteoarthritis in the rat model. Thirty male Wistar rats were divided into three groups (each of 10 rats). Rats of group I were injected with 1 mg MIA in the right ankle joint for two consecutive days, while those of group II were treated with saline instead of MIA; and group III (osteoarthritic +PRP) rats were injected with PRP in the ankle joint at 14, 21, and 28 days after MIA injection. Paw oedema, scoring of arthritis, Matrix metalloproteinase 13 (MMP13) level, antioxidant response element (ARE) level, and joint transforming growth factor beta1 (TGF β 1) were evaluated. PRP reduces expression of joint, MMP13, ARE level, and TGF β 1. PRP also significantly reduces the score of arthritis. The administration of PRP decreases paw oedema in MIA-induced OA rats. These results suggest that PRP has marked effect against OA in MIA-induced osteoarthritic rats which are mediated through the anti-inflammatory and antioxidant effects.
Keywords
ARE induced Osteoarthritis; MMP-13; Platelet-rich plasma; TGF β1
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