Evaluation of Transforming Growth Factor- β 1 Gene Expression and Polymorphism in Diagnosis of Hepatocellular Carcinoma in HCV-Infected Patients
Abdel Salam, F., Omar, M., Mohammed, S., El- Bahy, A. (2020). Evaluation of Transforming Growth Factor- β 1 Gene Expression and Polymorphism in Diagnosis of Hepatocellular Carcinoma in HCV-Infected Patients. EKB Journal Management System, 37(Special issue (Internal medicine and Hepatology)), 47-67. doi: 10.21608/bmfj.2020.111577
Fatma Abdel Salam; Maha Omar; Shuzan Mohammed; Abeer El- Bahy. "Evaluation of Transforming Growth Factor- β 1 Gene Expression and Polymorphism in Diagnosis of Hepatocellular Carcinoma in HCV-Infected Patients". EKB Journal Management System, 37, Special issue (Internal medicine and Hepatology), 2020, 47-67. doi: 10.21608/bmfj.2020.111577
Abdel Salam, F., Omar, M., Mohammed, S., El- Bahy, A. (2020). 'Evaluation of Transforming Growth Factor- β 1 Gene Expression and Polymorphism in Diagnosis of Hepatocellular Carcinoma in HCV-Infected Patients', EKB Journal Management System, 37(Special issue (Internal medicine and Hepatology)), pp. 47-67. doi: 10.21608/bmfj.2020.111577
Abdel Salam, F., Omar, M., Mohammed, S., El- Bahy, A. Evaluation of Transforming Growth Factor- β 1 Gene Expression and Polymorphism in Diagnosis of Hepatocellular Carcinoma in HCV-Infected Patients. EKB Journal Management System, 2020; 37(Special issue (Internal medicine and Hepatology)): 47-67. doi: 10.21608/bmfj.2020.111577

Evaluation of Transforming Growth Factor- β 1 Gene Expression and Polymorphism in Diagnosis of Hepatocellular Carcinoma in HCV-Infected Patients

Benha Medical Journal
Article 5, Volume 37, Special issue (Internal medicine and Hepatology), November 2020, Page 47-67  XML PDF (1.51 MB)
Document Type: Original Article
DOI: 10.21608/bmfj.2020.111577
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Authors
Fatma Abdel Salam1; Maha Omar1; Shuzan Mohammed2; Abeer El- Bahy3
1Department of Hepatology, Gastroenterology and Infectious Diseases Benha faculty of medicine, Benha University, Egypt.
2Department of Biochemistry, Benha faculty of medicine, Benha University, Egypt.
3Department of Hepatology, Gastroenterology and Infectious Diseases Benha faculty of medicine, Benha University, Egypt
Abstract
Background: Transforming growth factor-beta1 (TGF-β1), a member of transforming growth factor beta family, functions as a multi-functional cytokine and plays a key role in cellular growth, proliferation and differentiation. Aim of the work: To evaluate the role of TGF-β1 polymorphisms 509 C>T and TGF-β1 Gene expression in diagnosis of hepatocellular carcinoma (HCC) in cirrhotic Egyptian patients due to hepatitis C virus (HCV). Subjects and methods: Thirty five cirrhotic with HCC patients, thirty cirrhotic without HCC patients and 20 healthy volunteers were enrolled in this study. TGF-β1 gene expression in peripheral blood was quantitated using real-time polymerase chain reaction and molecular study of TGF-β1 509 C>T (rs1800469) polymorphism by Taqman allele discrimination was done for all subjects. Results: Showed that TGF-β1 -509 CT genotype was the most frequent genotype in  HCC patients and the most frequent allele was T allele in HCC patients, without significant difference of TGF-β1 -509 polymorphism in the studied groups. TGF-β1 gene expression was significantly higher in HCC patients (mean 3.19±1.8) than in cirrhotic without HCC patients (mean 1.76±0.95) (p < 0.001) and normal controls (mean 1.00 ±0.8) (p < 0.001), and AFP  ≥ 41 (ng/ml) and TGF-β1 gene expression  ≥ 1.85 are diagnostic for HCC presence. Conclusion: TGF-β1 gene expression in the peripheral blood may be used as a molecular marker for HCC diagnosis, and TGF-β1 polymorphism 509 C>T was not associated with the risk of HCC in HCV-cirrhotic patients.
Keywords
Transforming growth factor-beta 1 (TGF-β1); Hepatocellular carcinoma (HCC); Hepatitis C virus (HCV)
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