INFLUENCE OF FOLLICLE SIZE ON QUALITY OF CUMULUS OOCYTE-COMPLEXES AND CONCENTRATION OF STEROIDS OF FOLLICULAR FLUID IN BUFFALO
Heleil, B., Torner, H., Mahmoud,, S., Alm, H., Fattouh, E. (2003). INFLUENCE OF FOLLICLE SIZE ON QUALITY OF CUMULUS OOCYTE-COMPLEXES AND CONCENTRATION OF STEROIDS OF FOLLICULAR FLUID IN BUFFALO. EKB Journal Management System, 1(1), 345-362. doi: 10.21608/kvmj.2003.112225
B. Heleil; H. Torner; Sh.A. Mahmoud,; H. Alm; E.M Fattouh. "INFLUENCE OF FOLLICLE SIZE ON QUALITY OF CUMULUS OOCYTE-COMPLEXES AND CONCENTRATION OF STEROIDS OF FOLLICULAR FLUID IN BUFFALO". EKB Journal Management System, 1, 1, 2003, 345-362. doi: 10.21608/kvmj.2003.112225
Heleil, B., Torner, H., Mahmoud,, S., Alm, H., Fattouh, E. (2003). 'INFLUENCE OF FOLLICLE SIZE ON QUALITY OF CUMULUS OOCYTE-COMPLEXES AND CONCENTRATION OF STEROIDS OF FOLLICULAR FLUID IN BUFFALO', EKB Journal Management System, 1(1), pp. 345-362. doi: 10.21608/kvmj.2003.112225
Heleil, B., Torner, H., Mahmoud,, S., Alm, H., Fattouh, E. INFLUENCE OF FOLLICLE SIZE ON QUALITY OF CUMULUS OOCYTE-COMPLEXES AND CONCENTRATION OF STEROIDS OF FOLLICULAR FLUID IN BUFFALO. EKB Journal Management System, 2003; 1(1): 345-362. doi: 10.21608/kvmj.2003.112225

INFLUENCE OF FOLLICLE SIZE ON QUALITY OF CUMULUS OOCYTE-COMPLEXES AND CONCENTRATION OF STEROIDS OF FOLLICULAR FLUID IN BUFFALO

Kafrelsheikh Veterinary Medical Journal
Article 6, Volume 1, Issue 1, August 2003, Page 345-362  XML PDF (799.83 K)
Document Type: Original Article
DOI: 10.21608/kvmj.2003.112225
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Authors
B. Heleil* 1; H. Torner2; Sh.A. Mahmoud,3; H. Alm4; E.M Fattouh1
1Dept. of Theriogenology, Faculty of Veterinary Medicine, Tanta University
2Research Instit.Biology of Farm Animals, 18196 Dummerstorf, Germany.
3Dept. of Physiology, Faculty of Veterinary Medicine, Tanta University
4Research Instit.Biology of Farm Animals, 18196 Dummerstorf, Germany
Abstract
The aim of the present study was to investigate the effect of follicle size on the buffalo cumulus oocyte complexes (COCs) morphology, chromatin configuration, level of apoptosis in cumulus cells, and concentration of progesterone and estradiol-17b in buffalo follicular fluid of different follicle size. Visible follicles were carefully isolated and classified according to their size into 3 categories  5 mm (5-8 mm). A total of 295 COCs were recovered from the previous three follicle categories; COCs were classified according to number and compactness of cumulus layers. Good quality compact COCs which used for IVM, which mostly obtained from follicles
The chromatin configuration at the time of recovery in oocytes derived from 3-5 mm follicle respectively.
  There was a connection of cumulus morphology, chromatin configuration at the time of recovery and the follicle size.On the other hand no significant differences in maturation capacity (metaphase2)of COCs derived from different follicles diameter.
Apoptosis of cumulus at time of recovery decreased with incre-asing the follicle size, but this difference was not significant (0.9, 0.6,0.2). After 24 hr. IVM, an increase in apoptosis was observed in cumulus cells from all follicle size with significant (P < 0.05) increase in cumuli derived from 3-5 mm follicles compared to < 3 and >5 mm follicles (24.4 Vs 4.7,3.5)
Concentration of progesterone (ng/ml)in buffalo follicular fluid was not influenced by follicle size, while concentration of estradiol-17b (ng/ml) was significant higher in follicle 3-5, >5 mm (782 ± 67 and 788 ± 67 respectively) in comparison to follicular fluid from
Keywords
buffalo; oocyte; follicle size; apoptosis; progesterone; estradiol-17
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