ELECTROPHORETIC CHARACTERIZATION OF OUTER MEMBRANE PROTEIN EXTRACT FROM ESCHERICHIA COLI ISOLATED FROM GOAT MILK IN EGYPT | ||||
Kafrelsheikh Veterinary Medical Journal | ||||
Article 11, Volume 3, Issue 1, April 2005, Page 149-155 PDF (532.4 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/kvmj.2005.112454 | ||||
View on SCiNiTO | ||||
Authors | ||||
Hoda Abd El-Monem* 1; H.S. Nada2; S. M. Nada1 | ||||
1Animal Health Research Institute, Dokki, Egypt | ||||
2Faculty of Vet. Med., Tanata Univ., Kafr El-Sheikh branch | ||||
Abstract | ||||
Outer membrane protein (OMP) extracts of two E. coli strains O125 and O178 isolated from goat milk were purified and analyzed by use of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Each strain showed a distinct electrophoretic profile. Minor difference in profiles within strains showed the existence of different biotypes. It is concluded that SDS- PAGE may be used in the characterization and strain differentiation. The outer membrane protein extract from strain I revealed 2 distinct bands between 29.0 and 44.4 KD. On the other hand, the outer membrane protein extract for strain II revealed 3 distinct bands between the same range of molecular weight | ||||
Keywords | ||||
Outer membrane protein; E. coli strains | ||||
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