Molecular studies on antibiotic resistant Bacillus cereus isolated from meat products and human in Kaliobia, Egypt
Mousa, D., Abd El Tawab, A., El Hofy, F., Maarouf, A. (2020). Molecular studies on antibiotic resistant Bacillus cereus isolated from meat products and human in Kaliobia, Egypt. EKB Journal Management System, 38(2), 125-130. doi: 10.21608/bvmj.2020.25802.1187
Dina Mousa; Ashraf Abd El Tawab; Fatma El Hofy; Ahmed Maarouf. "Molecular studies on antibiotic resistant Bacillus cereus isolated from meat products and human in Kaliobia, Egypt". EKB Journal Management System, 38, 2, 2020, 125-130. doi: 10.21608/bvmj.2020.25802.1187
Mousa, D., Abd El Tawab, A., El Hofy, F., Maarouf, A. (2020). 'Molecular studies on antibiotic resistant Bacillus cereus isolated from meat products and human in Kaliobia, Egypt', EKB Journal Management System, 38(2), pp. 125-130. doi: 10.21608/bvmj.2020.25802.1187
Mousa, D., Abd El Tawab, A., El Hofy, F., Maarouf, A. Molecular studies on antibiotic resistant Bacillus cereus isolated from meat products and human in Kaliobia, Egypt. EKB Journal Management System, 2020; 38(2): 125-130. doi: 10.21608/bvmj.2020.25802.1187

Molecular studies on antibiotic resistant Bacillus cereus isolated from meat products and human in Kaliobia, Egypt

Benha Veterinary Medical Journal
Article 25, Volume 38, Issue 2, September 2020, Page 125-130  XML PDF (173.68 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2020.25802.1187
View on SCiNiTO View on SCiNiTO
Authors
Dina Mousa email 1; Ashraf Abd El Tawab2; Fatma El Hofy2; Ahmed Maarouf3
1Veterinarian
2Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University, Egypt.
3Animal Health Research Institute "Benha Branch". Egypt
Abstract
The current study was conducted on 210 random samples of meat products (beef burger, kofta, luncheon, minced meat, sausage) and diarrheic human stool of patients suffering from vomiting and diarrhoea (35 for each). The meat products were collected from different shops and hospitals at Kaliobia Governorate, Egypt, for detection of B. cereus strains, and their phenotypic characterization as well as antibiotic resistant genes. Bacteriological examination of the collected samples indicated the identification of 51 (24.3%) isolates of B. cereus from 210 samples as 11/31.4% from kofta 13/37.1% from minced meat; 9/25.7% from sausage; 7/20.0% from beef burger; 6 /17.1% from luncheon samples; and 5/14.3% from human stool specimens. Most of 51 isolated B. cereus strains had the ability for biofilm production. The antibiotic sensitivity profiles revealed that, the isolated B. cereus were highly resistant for Penicillin-G followed by methicillin; ampicillin; oxytetracycline; sulfa-trimethoprim and cefotaxime. Meanwhile, they were highly sensitive to to gentamycin and norfloxacin followed by ciprofloxacin; meropenem and florphenicol. Further, PCR declared that bla; tetA and erm genes were amplified in 9; 7; 6 out of 10 studied B. cereus isolates giving products of 680 bp.; 502 bp. and 645 bp. respectively. Therefore, one can conclude that; B. cereus especially antibiotic resistances ones are meat-borne pathogens of public health importance and they may be the causative agents in patients suffering from vomiting and diarrhoea.
Keywords
Meat products; B. cereus; antibiotic resistant genes; human
Statistics
Article View: 296
PDF Download: 385