TRICHINELLA SPIRALIS INFECTION PROTECTS AGAINST OVALBUMININDUCED ALLERGIC BRONCHIAL ASTHMA IN A MURINE MODEL | ||||
| Journal of the Egyptian Society of Parasitology | ||||
| Article 24, Volume 50, Issue 2, August 2020, Page 401-412 PDF (1.52 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jesp.2020.113071 | ||||
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| Authors | ||||
| NORHAN B. SALLAM1; SANAA N. ANTONIOS1; NEMA A. SOLIMAN2; DINA I. ELGENDY1 | ||||
| 1Department of Medical Parasitology | ||||
| 2Department of Medical Biochemistry | ||||
| Abstract | ||||
| Allergic bronchial asthma is a long-lasting illness of the respiratory system characterized by chronic inflammation of the respiratory airways with abnormal Th2-type immune responses to specific allergens. The accumulation of scientific evidence supports the beneficial effects of some parasitic infections in animal models of allergic diseases. Thus, the aim of this work was to discover the influence of infection with T. spiralis on ovalbumin-induced acute allergic airway inflammation in mice and to investigate whether this effect is correlated to the infection dose or not. Therefore, the severity of respiratory airways inflammation, the leukocyte counts in the blood and BALF, immunohistochemistry of FOXP3+CD4+T cells and the levels of IL-6, IL- 13, and IL-10 were assessed. In this experiment, 120 male laboratory bred mice were randomly divided into six groups: induced asthma, saline control, low dose preventive, high dose preventive, low dose therapeutic and high dose therapeutic groups. T. spiralis infection attenuated the intensity of pulmonary inflammation, decreased numbers of eosinophils in BALF and blood, reduced levels of IL-6 and IL-13 and elevated levels of IL-10 with a significant upregulation of FOXP3+CD4+T cells expression compared to the uninfected induced asthma group. The preventive groups showed the best results with no significant difference between effects of the low and the high doses. In conclusion, T. spiralis infection reduced Th2 type infammation and augmented regulatory immune response in ovalbumin-induced acute allergic airway inflammation with better preventive than therapeutic effects. Further investigations are needed to determine the minimal dose of infection that gives the best influence. | ||||
| Keywords | ||||
| Trichinella spiralis; Helminthic therapy; Ovalbumin; Acute allergic airway inflammation; IL-6; IL-13; IL-10; FOXP3+ CD4+T cells | ||||
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