ANTIVIRAL EFFECT OF THE EGYPTIAN BEE VENOM (BV) AND ITS FRACTION (PLA2) ON INFLUENZA VIRUS: IN-VITRO STUDY | ||||
| Journal of the Egyptian Society of Parasitology | ||||
| Article 25, Volume 50, Issue 2, August 2020, Page 413-422 PDF (772.48 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jesp.2020.113077 | ||||
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| Authors | ||||
| ABIR A. ELFIKY1; NORHAN M. ELASMY1; WALAA H. SALAMA2; AYMAN T. A. MORSY3 | ||||
| 1Anti-venom Research, (VACSERA), Dokki, Egypt | ||||
| 2Department of Molecular Biology; Genetic Engineering and Biotechnology Division, National Research Centre, Dokki | ||||
| 3Consultant Tropical Medicine and Fever | ||||
| Abstract | ||||
| Influenza is a viral infection that attacks the respiratory system. For most people, the flu disappears on its own but sometimes the flu and its complications can be deadly. This study established a new trend to treat Influenza virus by using bee venom and its fraction compared with the traditional flu drug. It was proved that bee venom (BV) has an antiviral effect. The bee venom (BV), bee venom phospholipase A2 (BV-PLA2) and ribovinal as a control drug were used. The cytotoxicity of the selected compounds was determined using MTT assay. Also, the cytopathic effect was measured after 24, 48 & 72 hours incubations and determination of gene expression of INF-gamma using SYBR Green. The IC50s of BV, BVPLA2 and ribovinal were 87.8, 17.3 &137.5μg/ml after 24 hours incubation, 36.9, 9.6 & 24.6μg/ml after 48 hours incubation, and 9.7, 7.45 & 31.6μg/ml after 72 hours incubation, respectively. In addition, the cytopathic effect of both BV and ribovinal was directly proportional to its concentration and incubation time. Whereas, BV-PLA2 showed an increase in cytopathic effect after 48 hours in spite of its concentration in case of direct antiviral effect. However, the indirect antiviral effect for both BV and PLA2 showed the same cytopathic effect in all concentrations and incubation times. So, the gene expression of INF-γ was significant increased after 48 hours incubation of either BV or ribovinal in direct and indirect effects. While the INF-γ expression was (14.8 & 16.4) direct and in indirect antiviral effects of BV-PLA2, respectively. These findings revealed the antiviral effect of both bee venom and BV-PLA2 against influenza virus. | ||||
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