PHYSIOLOGICAL IMPACTS OF DICHLOROPHYNYLDIMETHYL UREA ON SOME GREEN ALGAE SPECIES | ||||
| Egyptian Journal of Phycology | ||||
| Article 7, Volume 5, Issue 1, 2004, Page 97-105 PDF (298.81 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/egyjs.2004.113990 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Abo El-Khair B. El-Sayed; Mohamed M. El-Fouly; Abd El-Wahab A. Abdel-Maguid | ||||
| Fertilization Technology Department, National Research Centre | ||||
| Abstract | ||||
| Three algae species belonging to Chlorophyta; i.e Chlorella sp < /em>., Scenedesmus sp < /em>. and Haematococcus pluvialis, were incubated under different concentrations of the photosynthetic inhibitor dichlorophynyldimethylurea (DCMU). Algae were treated with 0, 1, 2, 4 and 8x10-5M DCMU added to N8 macronutrient solution containing ammonium acetate instead of potassium nitrate. The measured parameters were optical density, cell count, medium reaction (pH), total chlorophyll, total carotenoids, crude protein and lipids as ether extract. Results showed that DCMU inhibited growth as optical density for both Scenedesmus sp. and Chlorella sp. H. pluvialis exhibited more resistance against all given concentrations. Chlorophyll reached the maximum with lower and moderate levels of DCMU with Scenedesmus sp. and Chlorella sp. As for H. pluvialis, all used concentrations inhibited chlorophyll accumulation. A different manner was observed on carotenoids accumulation, where the decline of chlorophyll was associated with the decomposition of crude protein and rise in carotenoids and lipids content especially with H. pluvialis. | ||||
| Keywords | ||||
| Green algae; Chlorophyta; DCMU; optical density; cell count; total chlorophyll; carotenoids and biochemical metabolites | ||||
| References | ||||
|
Bose, S. and Hoch, G. E. (1981). P700 sensitization by low concentration of DCMU in isolated pea chloroplasts. Biochem. Biophys. Res. Common, 98: 541-547.
Doring, G.; Ringer, G.; Vaier, J. and Witt, H.T. (1969).Properties of the photo-protective chlorophyll a in photosynthesis. Z. Natureforsch, 24:1139-1143.
Duysens, L. N. M. and Amesz, J. (1962).Function and identification of two photochemical systems in photosynthesis. Biochem. Biophys. Acta, 64: 243-326.Fan, L.; Vonshak, A. and Boussiba, S. (1994).Effect of temperature and irradiance on growth of Haematococcus pluvialis (Chlorophyceae). J. Phycol., 38:829-833.
Gerovic, Z. G.; Langrand, E.; Latouche, G.; Morales, F. and Moya, I. (1998). Spectral characterization of NAD(P)H fluorescence in intact isolated chloroplasts and leaves: Effect of chlorophyll concentration on re-absorption of blue-green fluorescence. Phytosynthesis Research., 56 (3):291-301.
Haglund, K.; Ramazanov, Z.; Mtolera, M. and Pedersin, M. (1992). Role of external carbonic anhydrase in light-dependent alkalization by Fusca serratus L. and Laminaria saccharina (L) Lamur. (Pheophyta). Planta., 188: 1-6.
Helbust, J. A. and Carigie, J. S. (1983).Handbook of Physiological Studies. Cambridge Univ. Press : pp 64-70.
Izawa, S. (1968). Effect of Hill reaction inhibitors on photosystem I. In. K. Shibata, A. Takamia, A.T. Jagendorf and R. C Fuller. (eds). Comparative Biochemistry and Biophysics of Photosynthesis. Univeristy Press, State Collage, Pennsylvania, pp 140-147.
Kirilovsky D.; Rutherford, A. W. and Etienne, A. L. (1994). Influence of DCMU and ferricyanide on photo-damage in photosystem II. Biochemistry. Mar., 1533 (10): 3089-3095.
Ma, T.S. and Zauzage. C. (1942).Micro-kjeldahl determination of nitrogen, a new indicator and improved rapid method. Indust. Eng. Chem. Anal., 14: 280-286.
Nush, E. A. (1980).Comparison of different methods for chlorophyll and phaeophytin determination. Arch. Hydrobiol. Beih. Ergebn. Liminol, 14: 36.
Renger, G, (1973). The action of 3-(3,4 dichlorophynyl)-1,1-dimethylurea on the water splitting enzyme of photosynthesis. Biochem. Biophys. Acta., 314: 113-116.
Richmond, A. and Grobbelaar, J.U. (1986).Factor affecting the output rate of Spirulina platinesis with references to mass cultivation. Biomass, 10: 253-264.
Rosenberg, J.L.; Sahu, S . and Bigat, T.K. (1972). Quantum accumulation in photosynthetic oxygen evolution. Biophys. J., 12: 839-850.
Satory, D. P. (1982).Spectrophotometric analysis of chlorophyll a in fresh water phytoplankton. Technical report TRMS, Dept. of Environ. Affairs, Hydrologigal Res. Inst. Pretoria, R. S. A.
Shiraiwa, Y.; Goyal, A. and Tolbert, N. E. (1993). Alkalization of the medium by unicellular green algae during uptake of dissolved inorganic carbon. Plant Cell Physiol., 34(5): 649-657.
Soeder, C. J; Scholze, G. and Thiele, D. (1967). Einflulss Verschiedener Kultverbedingungen auf des wachstumn synchron kulturen von Chlorella jusca.S.H.et kr. Wasser Abwasser, 129(1):82-85.
Venkataraman, L. V. (1986).Blue green algae as bio-fertilizer. In: A. Richmond (ed.), CRC Handbook of Microalgal Mass Culture. CRC Press, Inc., 1986, pp: 445-471.
Wintermann, J. F. and de Mots. (1965). Spectrophotometric charactaristices of chlorophyll a and b and their phaeophytin in ethanol. Biochem. Biophys. Acta, 109: 448-453.
Zaborsky, O.R. (1985). Feeds from Spirulina: Process engineering and genetic engineering analysis of co-products. (OMEC International, Inc. Washington D.C). | ||||
|
Statistics Article View: 70 PDF Download: 54 |
||||
