EFFECT OF 2, 4-DICHLOROPHENOXY ACETIC ACID ON CYANOBACTERIA (ANABAENA SP) GROWTH IN THE PRESENCE OF EITHER GLOCUSE OR TRYPTOPHANE | ||||
| Egyptian Journal of Phycology | ||||
| Article 9, Volume 6, Issue 1, 2005, Page 127-135 PDF (421.5 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/egyjs.2005.114049 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Skina T. A. Tantawy1; El Sayeda A. Hassan2 | ||||
| 1Botany Department, Faculty of Sience, Cairo University, Giza, Egypt | ||||
| 2Agricultural Microbiology Department, Soils, Water & Environ. Res. Inst., Agric. Res. Center, Giza, Egypt | ||||
| Abstract | ||||
| 2,4-Dichiorophenoxyacetic acid (2,4-D) stimulated growth and heterocyst differentiation of Anabaena sp. in nitrogen free medium at low concentration 100 µg mL-1 while its higher concentrations inhibited both processes and 500 µg mL-1 proved to be lethal. Dry mass and specific growth rate of algae declined with increasing concentration of 2,4-D in the range of 100-1500 µg mL-1. Glucose slightly increased the heterocyst frequency without any lag in their differentiation. Tryptophan promoted growth of the alga and formation of heterocysts (nearly three fold). Tryptophan (50 µg mL-1) complex medium with 1 mg 2, 4-D per ml did not produce mature heterocysts. The filaments were fragmented at the point of heterocyst development and detached heterocyst germinated in situ. Glucose and tryptophan protected the alga, its growth and heterocyst differentiation even at the lethal concentration of the herbicide. | ||||
| References | ||||
|
Allen, M. B. and Arnon, D. I. (1955). Studies of the nitrogen-fixing blue-green algae. I. Growth and nitrogen fixation by Anabaena cylindrica Lemm. Plant Physiol., 30: 366-371.
Andreae, W. A. and Good, N. A. (1957). Studies in 3-indolacetic acid metabolism. Plant Physiol., 32:566-562.
Daw, M. M. and Than, T. (1981). The effect of blue green-algae biofertilizer, Azolla, triple superphosphate and urea supplied individually and in combination on the yield of rice. In Proceeding of Agricultural Research Congress Rangoon, Burma (360 paper) 14-23 February.
El-Kholy, M. H. (1997). Effect of soil irrigation levels, algalization and nitrogen application on rice and soil properties. Ph.D. Thesis, Soil Since, Department of Soil, Faculty of Agricultural, El-MansouraUniversity.
El-Nawawy, A. S.; Ibrahim, A. N. and Aboul-FadI, M. (1968). Nitrogen fixation by Calothrix sp. as influenced by certain sodium salts and nitrogenous compounds. Acta Agron. Acad. Sci. Hung., 17: 323-327.
Fay, P. (1973).The heterocysts, p. 238 In: The Biology of Blue-Green Algae (N.G. Carr, B.A. Whitton, eds). Blackwell, Oxford.
Fay, P.; Stewart, W. D. P.; Waisby, A. E., and Fogg, G. E. (1968). Is the heterocyst site of nitrogen fixation in blue-green algae? Nature, 220: 810.
Fogg, G. E. and Westlake, D. F. (1955). The importance of extracellular products of algae in fresh water. Int. Assoc. Theor. Appl. Limnol., 12: 219-225.
Ghazal, F. M. (1991). Cyanobacteria strains isolated from rice in Nile- Delta. In: The Blue-green algae collection. Inter. Nat. Rice Res. Inst. (IRRI), Manila, Philippines, June, 1991. p. 47.
Ismail, B. S.; Siew, T. C. and Mushrifah, I. (1995). Effect of molinate and carbofliran on nitrogen fixation by Azolla Pinnata. Microbes., 62: 127-134.
Kratz, W. A. and Myers, J. (1955). Nutrition and growth of several blue-green algae. Am. J. Bot., 42: 282-290.
Mitchison, G. J. and Wilcox, M. (1973). Alteration in heterocyst pattern of Anabaena produced by 7-aza-tryptophan. Nature New Biol., 246:229-232.
Moreland, D. E. (1980).Mechanisms of action of herbicides. Ann. Rev. Plant Physiol., 31: 597- 602.
Mumma, R. O. and Hamilton, R. H. (1976). Amino acid conjugates in Bound and Conjugated Pesticide Residues (D.D. Kaufman, G.G. Still, G.D. Paulson, S.K. Bandal, eds). American Chemical Society, Washington (DC); ACS Symp. Ser. 29: P 68.
Pandey, A. K. (1985). Effects of propanil on growth and cell constituents of Anabaena calcicola. Pesticide Biochem. Physiol., 23: 157-162.
Pandey, A. K. and Tiwari, D. N. (1993). Action of 2, 4-D on Nostoc linckia: Impact of glucose and tryptophan. Folia Microbiol., 31: 50-55.
Pandey, A. K.; Srivastava, V. and Trwari D. N. (1984). Toxicity of herbicide Stam f-34 (propanil) on Nostoc calcicola. Z. Allg. Mikrobiol., 24: 369-375.
Pandher, M. S.; Gurmehar, K.; Gosal, S. K.; Gupta, R. P. and Kauer, G. (1984). Effect of herbicides on the growth and nitrogen fixation by blue-green algae. Research and Development Reporter.,11: 9-13.
Pelroy, R. A.; Rippka, R. and Stanier, R. Y. (1972).The metabolism of glucose by unicellular blue-green algae. Arch. Mikrobiol., 87:303-310.
Seiler, J. P. (1978). The genetic toxicology of phenoxy acids other than 2, 4, 5-T. Mut. Res., 55: 197-206.
Singh, A. L.; Singh, P. K. and Singh, P. L. (1988). Effects of different herbicides on the Azolla and blue-green algal biofertilizer of rice. J. Agric. Sci., 111: 177-183.
Singh, H. N. and Vaishampayan, A. (1978). Biological effect of rice field herbicide machete on various strains of nitrogen fixing blue- green alga Nostoc muscorum. Environ. Exp. Bot., 18: 87-92.
Singh, R. N. (1961). Role of Blue-Green Algae in Nitrogen Economy of Indian Agriculture. Indian Council of Agricultural Research, New Delhi.
Srinivasan, G.; Pothiraj, P. and Choudhry, G. K. (1993).Response of Azolla (Azolla pinnata) to rice (Oriza sativa) herbicides. Ind. J. Agron., 38:156-158.
Stewart, W. D. P.; Hayastead, A. and Pearson, R. W. (1969). Nitrogenase activity in heterocyst of blue-green algae. Nature, 224: 227-230.
Tel-Or, E. and Stewart, W. D. P. (1977). Photosynthetic components and activities of nitrogen fixing isolated heterocyst of Anabaena cylindrica. Proc. Roy.Soc. London, Ser. B 193: P 61.
Tiwari, D. N.; Pandey, A. K. and Mishra, A. K. (1981).Action of 2, 4- dichloro.phenoxyacetic acid and rifampicin on heterocyst differentiation in the blue-green alga, Nostoc linckia. J. Biosci., 3: 33-40.
Tiwari, D. N.; Pandey, A. K.; Mishra, A. K. and Srivastava, V. (1982). Role of 2, 4-D on growth and macromolecular synthesis in a cyanobacterium, Anabaena cylindrica. Ind. J. Bot., 5: 111-116.
Vaishampayan, A. (1982). Cu-Fe interaction in the N2 -fixing cyano bacterium Nostoc muscorum. Microbios Letters, 21: 17-23.
Vaishampayan, A.; Singh, H. R. and Singh, U. N. (1982). Biological effect of rice-field herbicide Stam f-34 on various strains of nitrogen-fixing blue-green alga Nostoc muscorum. Biochem. Physiol. Pflanzen., 173: 410- 417.
Watanabe, A. and Yamamoto, Y. (1971). Algal nitrogen-fixation in the tropics. Plant and Soil, 160: 403-413.
Watanabe, I. and Liu, C. C. (1992). Improving nitrogen-fixing system and integrating them into sustainable rice farm. Plant and Soil, 141: 57-67. | ||||
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