Effect of Vitamin C Pre-treatment on Germination, Seedling Growth and some Metabolic Activities of kidney bean (Phaseolus vulgaris L) under Different Temperatures. | ||||
| Egyptian Journal of Botany | ||||
| Article 6, Volume 56, Issue 2, February 2016, Page 439-449 PDF (501.51 K) | ||||
| DOI: 10.21608/ejbo.2016.1144 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| Hanan. M. Abou El-Ghit* | ||||
| Department of Botany and Microbiology, Faculty of Science, University of Helwan, | ||||
| Abstract | ||||
| VITAMIN C pretreatments showed pronounced mitigating effects …..on kidney bean under different temperatures, where the germination %, seedling gr owth and vigor were significantly increased, compared with the corresponding results obtained under exposure to different temperatures without vitamin C pretreatments. A similar trend was shown with regard to total chlorophyll, total carbohydrate and soluble protein contents. Lower temperatures as well as vitamin C pretreatments led to higher accumulation of soluble sugar contents in seedlings. Proline content was considerably increased by lower temperature levels and vitamin C pretreatments also enhanced its increase. Vitamin C was assumed to act synergistically with lower temperatures in decreasing proline dehydrogenase level. The activity levels of some hydrolytic enzymes and antioxidant enzymes were affected by both lower temperatures and vitamin C treatments. Thus, amylase and protease activities were significantly increased by lower temperatures and vitamin C pretreatments. Catalase and ascorbate peroxidase activities were remarkably elevated by decreasing temperature but were decreased as a result of vitamin C pretreatments. Higher vitamin C levels were more effective in alleviating the adverse effects of lower temperature on the above mentioned criteria of the kidney bean. | ||||
| Keywords | ||||
| kidney bean; Germination; Temperature; Chlorophyll; proline; Antioxidant enzymes; hydrolytic enzymes | ||||
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