Alpha-Amylase Enzymes of Tribolium castaneum (Herbst) and Callosobruchus maculatus (F.) And Their Response to A-Amylase Inhibitor from Rice أنزبم الألفا أميليز فى حشرتى خنفساء الدقيق الحمراء Tribolium castaneum (Herbst) وخنفساء اللوبيا Callosobruchus maculatus (F.) واستجابتهم لمثبط ألفا أميليز من الأرز | ||||
| Journal of Plant Protection and Pathology | ||||
| Article 8, Volume 11, Issue 8, August 2020, Page 421-426 PDF (1.26 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jppp.2020.114589 | ||||
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| Authors | ||||
Ashraf Oukasha Abd El-latif  ; Nessma Mohieldeen; Ahmed Mahmoud Salman
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| Department of Plant protection, Faculty of Agriculture, Sohag University, Egypt | ||||
| Abstract | ||||
| Insect α-amylase has recently become a target of insect control strategies using plant-derived α-amylase inhibitors especially for the control of stored product insect pests. The first step to establish such strategy is to characterize the α-amylase enzymes in the target pest. In the current study, α-amylase was biochemically characterized in the larval and adult stages of Tribolium castaneum and Callosobruchus maculatus. T. castaneum was found to have higher α-amylase activity with lower temperature stability compared to that of C. maculatus. The optimum pH for α-amylase activity was 5-6. Zymogram pattern revealed the presence of two α-amylase isoforms with high molecule weight in T. castaneum. C. maculatus has two α-amylase isoforms in the adult stage and three isoforms in the larval stage. Rice α-amylase inhibitors was purified and found to have strong inhibitory effect against α-amylase of both insects and also negatively affected their life parameters. | ||||
| Keywords | ||||
| Tribolium castaneum; Callosobruchus maculatus; α-amylase; α-amylase inhibitors | ||||
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