Biological and Molecular Studies on an Egyptian Potyvirus Isolate from Hyocyamus muticus L. | ||||
| Egyptian Journal of Botany | ||||
| Article 9, Volume 56, Issue 2, February 2016, Page 489-505 PDF (736.77 K) | ||||
| DOI: 10.21608/ejbo.2017.1147 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Reham1* M. Elbaz* 1; E.T. Abd El-Salam2; M.E. Osman3; Asmaa F. Abd El-Monem3 | ||||
| 1Botany and Microbiology Department, Faculty of Science, Helwan University. | ||||
| 2Botany and Microbiology Department, Faculty of Science, Cairo University, | ||||
| 3Botany and Microbiology Department, Faculty of Science, Helwan University | ||||
| Abstract | ||||
| ANEW isolate of potyvirus was isolated from naturally-infected ……. henbane plants (Hyocyamus muticus L.) collected from the farm of Faculty of Pharmacy, Cairo University in January 2013. The naturally infected henbane plants exhibited severe mosaic, rugosity, blistering and malformation. Thermal inactivation point, dilution end point and longevity in vitro of virus isolate were found to be 65 ºC, 10- 4 and 4 days, respectively. Electron micrograph of partial purified virus negat ively s t ained wit h 2% p hosp hot unges t ic acid revealed the presence of filamentous viruses with size 1440 X14.3nm. Total RNA was extracted from infected henbane plant. Comparative nucleotide sequence analysis for virus showed a high degree of similarity (62 %) with four potyviruses accessions (EU482153: Potato virus Y isolate Foggia, KF850513: Potato virus Y isolate M3, AM184113: Henbane mosaic virus partial gene for polyprotein- PHYS/H isolate, AY166867: Potato virus Y strain N isolate). The virus isolate was published in GenBank with accession number KM497011. | ||||
| Keywords | ||||
| Potyvirus; electron microscopy; Comparative Nucleotide sequence | ||||
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